Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method

被引：0
|
作者：
Man, Peng [1 ,3 ]
Bai Zhongchen [2 ]
Ying, Zhang [2 ]
Li Xianji [2 ]
Zhang Zhengping [2 ]
机构：
[1] Guizhou Univ, Coll Big Data & Informat Engn, Guiyang 550025, Guizhou, Peoples R China
[2] Guizhou Univ, Key Lab Photoelect & Applicat Technol, Guiyang 550025, Guizhou, Peoples R China
[3] Guizhou Normal Univ, Sch Mech & Elect Engn, Guiyang 550025, Guizhou, Peoples R China
来源：
LASER & OPTOELECTRONICS PROGRESS | 2019年 / 56卷 / 06期
关键词：
laser technique; carboxyl quantum clots; fluorescence quenching method; nerve growth factor; antigen; antibody; FABRICATION;
D O I：
10.3788/LOP56.062601
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
A new method for detecting the nerve growth factors (NGF) is proposed based on the fluorescence quenching effect of carboxyl quantum clots. The research results show that in the pH7.5 buffer solution, the NGF antigen specifically binds to the carboxyl quantum dot-labeled NGF antibody, which can quench the fluorescence of the carboxyl quantum clots. The fluorescence quenching degree and the NGF antigen mass concentration has a good linear relationship in the range of 1-20 ng/mL, and the linear correlation coefficient is 0.9696 with the detection limit of 1 ng/ml,. This proposed method has good anti-interference characteristics and has great potential applications in diagnosis and detection.
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页数：7
相关论文
共 26 条
  • [1] Nerve growth factor: role in growth, differentiation and controlling cancer cell development
    Aloe, Luigi
    Rocco, Maria Luisa
    Balzamino, Bijorn Omar
    Micera, Alessandra
    [J]. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2016, 35
  • [2] Bai ZC, 2017, CHINESE J LASERS, V44
  • [3] Current and emerging commercial optical biosensors
    Baird, CL
    Myszka, DG
    [J]. JOURNAL OF MOLECULAR RECOGNITION, 2001, 14 (05) : 261 - 268
  • [4] Phase I Trial of Recombinant Human Nerve Growth Factor for Neurotrophic Keratitis
    Bonini, Stefano
    Lambiase, Alessandro
    Rama, Paolo
    Filatori, Isabella
    Allegretti, Marcello
    Chao, Wendy
    Mantelli, Flavio
    [J]. OPHTHALMOLOGY, 2018, 125 (09) : 1468 - 1471
  • [5] Chen F T., 2009, RES DIAGNOSIS EARLY, P12
  • [6] Chen W B, 2017, LASER OPTOELECTRONIC, V54
  • [7] A new nerve growth factor-mimetic peptide active on neuropathic pain in rats
    Colangelo, Anna Maria
    Bianco, Maria Rosaria
    Vitagliano, Luigi
    Cavaliere, Carlo
    Cirillo, Giovanni
    De Gioia, Luca
    Diana, Donatella
    Colombo, Daniele
    Redaelli, Cristina
    Zaccaro, Laura
    Morelli, Giancarlo
    Papa, Michele
    Sarmientos, Paolo
    Alberghina, Lilia
    Martegani, Enzo
    [J]. JOURNAL OF NEUROSCIENCE, 2008, 28 (11): : 2698 - 2709
  • [8] Association of brain-derived neurotrophic factor and nerve growth factor gene polymorphisms with susceptibility to migraine
    Coskun, Salih
    Varol, Sefer
    Ozdemir, Hasan H.
    Agacayak, Elif
    Aydin, Birsen
    Kapan, Oktay
    Camkurt, Mehmet Akif
    Tunc, Saban
    Cevik, Mehmet Ugur
    [J]. NEUROPSYCHIATRIC DISEASE AND TREATMENT, 2016, 12 : 1779 - 1785
  • [9] Use of Luminex xMAP-derived Bio-Plex bead-based suspension array for specific detection of PPV W and characterization of epitopes on the coat protein of the virus
    Croft, Heather
    Malinowski, Tadeusz
    Krizbai, Laszlo
    Mikec, Ivan
    Kajic, Vesna
    Reed, Christopher
    Varga, Aniko
    James, Delano
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2008, 153 (02) : 203 - 213
  • [10] 崔大付, 2003, [自然科学进展, Progress in Natural Science], V13, P874
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