Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method

被引：0
作者：
Man, Peng [1 ,3 ]
Bai Zhongchen [2 ]
Ying, Zhang [2 ]
Li Xianji [2 ]
Zhang Zhengping [2 ]
机构：
[1] Guizhou Univ, Coll Big Data & Informat Engn, Guiyang 550025, Guizhou, Peoples R China
[2] Guizhou Univ, Key Lab Photoelect & Applicat Technol, Guiyang 550025, Guizhou, Peoples R China
[3] Guizhou Normal Univ, Sch Mech & Elect Engn, Guiyang 550025, Guizhou, Peoples R China
来源：
LASER & OPTOELECTRONICS PROGRESS | 2019年 / 56卷 / 06期
关键词：
laser technique; carboxyl quantum clots; fluorescence quenching method; nerve growth factor; antigen; antibody; FABRICATION;
D O I：
10.3788/LOP56.062601
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
A new method for detecting the nerve growth factors (NGF) is proposed based on the fluorescence quenching effect of carboxyl quantum clots. The research results show that in the pH7.5 buffer solution, the NGF antigen specifically binds to the carboxyl quantum dot-labeled NGF antibody, which can quench the fluorescence of the carboxyl quantum clots. The fluorescence quenching degree and the NGF antigen mass concentration has a good linear relationship in the range of 1-20 ng/mL, and the linear correlation coefficient is 0.9696 with the detection limit of 1 ng/ml,. This proposed method has good anti-interference characteristics and has great potential applications in diagnosis and detection.
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页数：7
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