The assessment of the quality of the graft in an animal model for lung transplantation using the metabolomics 1H high-resolution magic angle spinning NMR spectroscopy

被引:21
作者
Benahmed, Malika A. [2 ,3 ]
Santelmo, Nicola [4 ]
Elbayed, Karim [3 ]
Frossard, Nelly [5 ]
Noll, Eric [6 ]
Canuet, Mathieu [7 ]
Pottecher, Julien [8 ,9 ]
Diemunsch, Pierre [6 ]
Piotto, Martial [10 ]
Massard, Gilbert [4 ]
Namer, Izzie J. [1 ,2 ]
机构
[1] Hop Hautepierre, Hop Univ Strasbourg, Serv Biophys & Med Nucl, F-67098 Strasbourg, France
[2] Univ Strasbourg, CNRS UMR7237, LINC, Fac Med,Inst Phys Biol, Strasbourg, France
[3] Univ Strasbourg, CNRS UMR7177, Lab RMN & Biophys Membranes, Inst Chim, Strasbourg, France
[4] Nouvel Hop Civil, Hop Univ Strasbourg, Serv Chirurg Thorac, Strasbourg, France
[5] Univ Strasbourg, CNRS UMR 7200, Fac Pharm, Illkirch Graffenstaden, France
[6] Hop Hautepierre, Hop Univ Strasbourg, Anesthesiol Serv, F-67098 Strasbourg, France
[7] Nouvel Hop Civil, Hop Univ Strasbourg, Serv Pneumol, Strasbourg, France
[8] Nouvel Hop Civil, Hop Univ Strasbourg, Serv Reanimat Chirurg, Strasbourg, France
[9] Univ Strasbourg, Fac Med, Inst Physiol, EA3072, Strasbourg, France
[10] Bruker Biospin, Wissenbourg, France
关键词
lung transplantation; graft quality; HRMAS; NMR spectroscopy; metabolomics; GLUTATHIONE; HEART; PRESERVATION;
D O I
10.1002/mrm.24110
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Standards are needed to control the quality of the lungs from nonheart-beating donors as potential grafts. This was here assessed using the metabolomics 1H high-resolution magic angle spinning NMR spectroscopy. Selective perfusion of the porcine bilung block was set up 30 min after cardiac arrest with cold Perfadex (R). Lung alterations were analyzed at 3, 6, and 8 h of cold ischemia as compared to baseline and to nonperfused lung. Metabolomics analysis of lung biopsies allowed identification of 35 metabolites. Levels of the majority of the metabolites increased over time at 4 degrees C without perfusion, indicating cellular degradation, whereas levels of glutathione decreased. When lung was perfused at 4 degrees C, levels of the majority of the metabolites remained stable, including levels of glutathione. Levels of uracil by contrast showed a reverse profile, as its signal increased over time in the absence of perfusion while being totally absent in perfused samples. Our results showed glutathione and uracil as potential biomarkers for the quality of the lung. The metabolomics 1H high-resolution magic angle spinning NMR spectroscopy can be efficiently applied for the assessment of the quality of the lung as an original technique characterized by a rapid assessment of intact biopsy samples without extraction and can be implemented in hospital environment. Magn Reson Med, 2012. (c) 2011 Wiley Periodicals, Inc.
引用
收藏
页码:1026 / 1038
页数:13
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