SAXS Study of Sterically Stabilized Lipid Nanocarriers Functionalized by DNA

被引：9

作者：

Angelov, Borislav ^{[1
]}

Angelova, Angelina ^{[2
]}

Filippov, Sergey ^{[1
]}

Karlsson, Goran ^{[3
]}

Terrill, Nick ^{[4
]}

Lesieur, Sylviane ^{[2
]}

Stepanek, Petr ^{[1
]}

机构：

[1] ASCR, Inst Macromol Chem, Prague, Czech Republic

[2] Univ Paris Sud, CNRS, UMR8612, Phys Chimie Pharmacotech Biopharm, Chatenay Malabry, France

[3] Uppsala Univ, Dept Phys & Analyti Chem, Uppsala, Sweden

[4] Diamond Light Source Ltd, Oxford OX11 0DE, England

来源：

SANS-YUMO USER MEETING AT THE START-UP OF SCIENTIFIC EXPERIMENTS ON THE IBR-2M REACTOR: DEVOTED TO THE 75TH ANNIVERSARY OF YU M OSTANEVICH'S BIRTH | 2012年 / 351卷

关键词：

X-RAY-SCATTERING; CATIONIC LIPOSOMES; GENE DELIVERY; NEUROTROPHIC FACTOR; PHASE-TRANSITION; CUBIC PHASE; COMPLEXES; NANOPARTICLES; VESICLES; TRANSFECTION;

D O I：

10.1088/1742-6596/351/1/012004

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The structure of novel spontaneously self-assembled plasmid DNA/lipid complexes is investigated by means of synchrotron radiation small-angle X-ray scattering (SAXS) and Cryo-TEM imaging. Liquid crystalline (LC) hydrated lipid systems are prepared using the nonionic lipids monoolein and DOPE-PEG(2000) and the cationic amphiphile CTAB. The employed plasmid DNA (pDNA) is encoding for the human protein brain-derived neurotrophic factor (BDNF). A coexistence of nanoparticulate objects with different LC inner organizations is established. A transition from bicontinuous membrane sponges, cubosome intermediates and unilamelar liposomes to multilamellar vesicles, functionalized by pDNA, is favoured upon binding and compaction of pBDNF onto the cationic PEGylated lipid nanocarriers. The obtained sterically stabilized multicompartment nanoobjects, with confined supercoiled plasmid DNA (pBDNF), are important in the context of multicompartment lipid nanocarriers of interest for gene therapy of neurodegenerative diseases.

引用

页数：9

共 41 条

[1] Stabilized plasmid-lipid particles containing PEG-diacylglycerols exhibit extended circulation lifetimes and tumor selective gene expression [J].