Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction

被引:22
作者
Falcone, E [1 ]
DAmore, E [1 ]
DiTrani, L [1 ]
Sili, A [1 ]
Tollis, M [1 ]
机构
[1] IST SUPER SANITA,LAB MED VET,I-00161 ROME,ITALY
关键词
infectious bronchitis virus; polymerase chain reaction; diagnosis;
D O I
10.1016/S0166-0934(96)02151-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:125 / 130
页数:6
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