Development of glucose oxidase-immobilized alginate nanoparticles for enhanced glucose-triggered insulin delivery in diabetic mice

被引：34
作者：
Chai, Zhihua [1 ]
Dong, Huiyu [1 ]
Sun, Xiaoyu [1 ]
Fan, Yiting [1 ]
Wang, Yanxia [1 ]
Huang, Fan [2 ]
机构：
[1] North China Inst Sci & Technol, Dept Environm Engn, POB 206, Beijing 101601, Peoples R China
[2] Chinese Acad Med Sci & Peking Union Med Coll, Inst Radiat Med, Tianjin Key Lab Radiat Med & Mol Nucl Med, Beijing, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 159卷
基金：
中国国家自然科学基金;
关键词：
Sodium alginate; Phenylboronic acid; Nanoparticles; ACID-FUNCTIONALIZED NANOPARTICLES; PHENYLBORONIC ACID; CHITOSAN NANOPARTICLES; COMPLEXATION; SENSITIVITY; RELEASE; PH; FABRICATION; HYDROGEL; PATCHES;
D O I：
10.1016/j.ijbiomac.2020.05.097
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work, we successfully developed poly(acrylamido phenylboronic acid)/sodium alginate nanoparticles (NPs) via formation of cycloborates (glucose- and H2O2-responsive functional groups), as an improved glucose-mediated insulin delivery system loaded with glucose oxidase (GOx). Dynamic light scattering revealed that the GOx-loaded NPs showed better glucose-sensitivity than the GOx-unloaded NPs. In addition, compared to insulin-loaded NPs, the insulin/GOx-loaded NPs displayed faster glucose-responsive insulin release. Importantly, there was a significant hypoglycemic effect on diabetic mice following the subcutaneous injection of insulin/GOx-loaded NPs. Furthermore, the NPs exhibited favorable biocompatibility as demonstrated by cytotoxicity assay, hemolysis study, and histopathological examination. The NPs have the advantages of easy preparation, enhanced glucose-responsiveness, and good biocompatibility, making them as potential candidates for subcutaneous insu-lin delivery. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码：640 / 647
页数：8
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