Structural characterization and fine chromosomal mapping of the human P2Y(1) purinergic receptor gene (P2RY(1))

Using P2Y(1) specific oligonucleotide primers in a Polymerase Chain Reaction on human genomic DNA, we have amplified a region encoding the P2Y(1) receptor: Restriction analysis and Southern hybridization of the PCR product revealed that the entire open reading frame of the human P2Y(1) receptor is coded by an intronless gene. We have previously localized the P2Y(1) receptor gene to human chromosome 3. The gene was further localized to a region of chromosome 3 using a subchromosomal hybrid panel containing differ-ent segments of chromosome 3. Based on the specific PCR product obtained and its Southern hybridization to the human P2Y(1) receptor cRNA, the P2Y(1) receptor gene was mapped to human chromosome 3q25.