SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1)

被引：60

作者：

Prinzenberg, EM ^{[1
]}

Krause, I ^{[1
]}

Erhardt, G ^{[1
]}

机构：

[1] Univ Giessen, Dept Anim Breeding & Genet, D-35390 Giessen, Germany

来源：

ANIMAL BIOTECHNOLOGY | 1999年 / 10卷 / 1-2期

关键词：

kappa-casein polymorphism; CSN3; SSCP;

D O I：

10.1080/10495399909525921

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H and I), and the third (named CSN3*A(1)) was found in unrelated animals, thus indicating the presence of three additional alleles at the bovine CSN3 locus. DNA sequencing revealed single mutations that led to a Thr/Ile substitution in amino acid position 135 for CSN3*H and to a Ser/Ala substitution in position 104 of the deduced amino acid sequence of CSN3*I (GenBank accession numbers AF105260 and AF121023) compared to CSN3*A. In CSN3*A(1), a silent mutation in the third codon position of Pro(150) was found (GenBank accession number AF092513).

引用

页码：49 / 62

页数：14

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