ELECTRICAL CHARACTERIZATION OF SEQUENCE-SPECIFIC LABEL-FREE DNA BY USING POLYSILICON WIRE

被引：0

作者：

Wu, You-Lin ^{[1
]}

Hsu, Po-Yen ^{[1
]}

Hsu, Chung-Ping ^{[2
]}

Liu, Wen-Cheng ^{[3
]}

机构：

[1] Natl Chi Nan Univ, Dept Elect Engn, Nantou, Taiwan

[2] Taichung Vet Gen Hosp, Div Chest Surg, Dept Surg, Taichung, Taiwan

[3] Natl Chi Nan Univ, Grad Inst Biomed & Biomed, Nantou, Taiwan

来源：

BIODEVICES 2010: PROCEEDINGS OF THE THIRD INTERNATIONAL CONFERENCE ON BIOMEDICAL ELECTRONICS AND DEVICES | 2010年

关键词：

DNA bases detection; Polysilicon wire sensor; Label-free; MOLECULES;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper we report the electrical measurement of sequence-specific and label-free deoxyribonucleic acid (DNA) by using poly-silicon (poly-Si) wire. Four single-strained (ss) DNA bases, adenine (A), thymine (T), cytosine (C) and guanines (G), as well as double-stranded (ds) DNA sequences, ds(A-T) and ds(C-G), with different lengths and concentrations were dropped onto the poly-Si wire surface, and currents flowing through the poly-Si wire channel were determined. It is found that the amount change of the channel current AI for ssDNAs with fixed length and fixed concentration is that T > C > G > A. For dsDNA sequence, we observe that ds(A-T) has higher AI than does ds(C-G). We also prove that single base change in ssDNA is feasible by using the poly-Si wire sensor.

引用

页码：170 / 173

页数：4

共 12 条

[1] Current strategies for electrochemical detection of DNA with solid electrodes [J].