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RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection (Retracted Article)
被引:24
作者:
Chan, Che-Man
Woo, Patrick C. Y.
[2
]
Lau, Susanna K. P.
[2
]
Tse, Herman
[2
]
Chen, Hong-Lin
Li, Feng
[3
]
Zheng, Bo-Jian
[2
]
Chen, Ling
[3
]
Huang, Jian-Dong
[4
]
Yuen, Kwok-Yung
[1
,2
]
机构:
[1] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 501663, Guangdong, Peoples R China
[4] Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
关键词:
coronavirus HKU1;
spike;
maturation;
glycoprotein;
N-glycosylation;
furin inhibitor;
surface expression;
native conformation;
life cycle;
seroepidemiology;
virus maturation;
D O I:
10.3181/0806-RM-197
中图分类号:
R-3 [医学研究方法];
R3 [基础医学];
学科分类号:
1001 ;
摘要:
We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Exp Biol Med 233:1527-1536, 2008
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页码:1527 / 1536
页数:10
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