RETRACTED: Spike Protein, S, of Human Coronavirus HKU1: Role in Viral Life Cycle and Application in Antibody Detection (Retracted Article)

被引:24
作者
Chan, Che-Man
Woo, Patrick C. Y. [2 ]
Lau, Susanna K. P. [2 ]
Tse, Herman [2 ]
Chen, Hong-Lin
Li, Feng [3 ]
Zheng, Bo-Jian [2 ]
Chen, Ling [3 ]
Huang, Jian-Dong [4 ]
Yuen, Kwok-Yung [1 ,2 ]
机构
[1] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 501663, Guangdong, Peoples R China
[4] Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
关键词
coronavirus HKU1; spike; maturation; glycoprotein; N-glycosylation; furin inhibitor; surface expression; native conformation; life cycle; seroepidemiology; virus maturation;
D O I
10.3181/0806-RM-197
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Exp Biol Med 233:1527-1536, 2008
引用
收藏
页码:1527 / 1536
页数:10
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