Progressive Transverse Microtubule Array Organization in Hormone-Induced Arabidopsis Hypocotyl Cells

被引:64
作者
Vineyard, Laura [1 ]
Elliott, Andrew [1 ]
Dhingra, Sonia [1 ]
Lucas, Jessica R. [2 ]
Shaw, Sidney L. [1 ]
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
[2] Santa Clara Univ, Dept Biol, Santa Clara, CA 95053 USA
基金
美国国家科学基金会;
关键词
CORTICAL MICROTUBULES; GAMMA-TUBULIN; EPIDERMAL-CELLS; DYNAMIC MICROTUBULES; SELF-ORGANIZATION; GROWTH; GIBBERELLIN; ORIENTATION; NUCLEATION; ALIGNMENT;
D O I
10.1105/tpc.112.107326
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The acentriolar cortical microtubule arrays in dark-grown hypocotyl cells organize into a transverse coaligned pattern that is critical for axial plant growth. In light-grown Arabidopsis thaliana seedlings, the cortical array on the outer (periclinal) cell face creates a variety of array patterns with a significant bias (>3:1) for microtubules polymerizing edge-ward and into the side (anticlinal) faces of the cell. To study the mechanisms required for creating the transverse coalignment, we developed a dual-hormone protocol that synchronously induces; 80% of the light-grown hypocotyl cells to form transverse arrays over a 2-h period. Repatterning occurred in two phases, beginning with an initial 30 to 40% decrease in polymerizing plus ends prior to visible changes in the array pattern. Transverse organization initiated at the cell's midzone by 45 min after induction and progressed bidirectionally toward the apical and basal ends of the cell. Reorganization corrected the edge-ward bias in polymerization and proceeded without transiting through an obligate intermediate pattern. Quantitative comparisons of uninduced and induced microtubule arrays showed a limited deconstruction of the initial periclinal array followed by a progressive array reorganization to transverse coordinated between the anticlinal and periclinal cell faces.
引用
收藏
页码:662 / 676
页数:15
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