Cytosolic reducing power preserves glutamate in retina

被引：51

作者：

Du, Jianhai ^{[1
]}

Cleghorn, Whitney ^{[1
]}

Contreras, Laura ^{[5
,6
]}

Linton, Jonathan D. ^{[1
]}

Chan, Guy C. -K. ^{[2
]}

Chertov, Andrei O. ^{[1
]}

Saheki, Takeyori ^{[7
]}

Govindaraju, Viren ^{[1
]}

Sadilek, Martin ^{[3
]}

Satrustegui, Jorgina ^{[5
,6
]}

Hurley, James B. ^{[1
,4
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA

[3] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[4] Univ Washington, Dept Ophthalmol, Seattle, WA 98195 USA

[5] Univ Autonoma Autonoma Madrid, Consejo Super Invest Cient, Dept Biol Mol, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain

[6] Univ Autonoma Autonoma Madrid, Consejo Super Invest Cient, Ctr Invest Biomed Red Enfermedades Raras, Madrid 28049, Spain

[7] Kumamoto Univ, Inst Resource Dev & Anal, Kumamoto 8600811, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 46期

基金：

美国国家卫生研究院;

关键词：

ASPARTATE-GLUTAMATE; ENERGY-METABOLISM; CYCLING ASSAY; DEGENERATION; GLUCOSE; MITOCHONDRIA; CARRIER; ARALAR; RABBIT; GROWTH;

D O I：

10.1073/pnas.1311193110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glutamate in neurons is an important excitatory neurotransmitter, but it also is a key metabolite. We investigated how glutamate in a neural tissue is protected from catabolism. Flux analysis using C-13-labeled fuels revealed that retinas use activities of the malate aspartate shuttle to protect >98% of their glutamate from oxidation in mitochondria. Isolation of glutamate from the oxidative pathway relies on cytosolic NADH/NAD(+), which is influenced by extracellular glucose, lactate, and pyruvate.

引用

页码：18501 / 18506

页数：6

共 28 条

[1] DISTRIBUTION OF GLUCOSE AND LACTATE IN THE INTERPHOTORECEPTOR MATRIX [J].