Effects of antisense glutamic aciddecarboxylase oligodeoxynucleotide on epileptic rats induced by pentylenetetrazol

被引：0

作者：

He, XH ^{[1
]}

Wang, W

Ruan, XZ

Li, WX

Zhang, L

机构：

[1] Wuhan Univ, Sch Life Sci, Dept Virol & Mol Biol, Wuhan 430070, Peoples R China

[2] Tongji Med Univ, Tongji Hosp, Dept Neurol, Wuhan 430030, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2002年 / 115卷 / 03期

关键词：

epilepsy; glutamic acid decarboxylase; oligodeoxynucleotide; pentylenetetrazol;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective To investigate the effects of antisense glutamic acid decarboxylase (GAD(67)) oligodeoxynucleotide ( ODN) on behavior, seizure threshold and EEG of hippocampus in the epileptic rats induced by pentylenetetrazol (PTZ). Methods A model of chronic epilepsy in rats was established by PTZ. The inhibition of GAD(67) mRNA expression in hippocampus was selectively induced by antisense oligodeoxynucleotide of GAD(67). The effect of antisense GAD(67) ODN on behavior, seizure threshold and EEG recording of kindled rats was examined. Results Antisense GAD(67) ODN could inhibit the expression of GAD(67) mRNA and the concentration of GABA. It also could significantly shorten the latencies of seizure and increase the level of seizure and the frequency of epileptiforrn discharges. Conclusion The gene of GAD(67) may be an anti-seizure gene, which might inhibit epileptiform discharge. The treatment of epilepsy by GAD(67) gene will have a bright future.

引用

页码：425 / 429

页数：5

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