Isolation and Characterization of Pepsin-Soluble Collagen from Abalone (Haliotis discus hannai) Gastropod Muscle Part II

Abalone (Haliotis discus hannai) gastropod muscle was divided into Part I (the center part of gastropod muscle) and Part II (the transforming and virginal part of gastropod muscle). Pepsin-soluble collagen was extracted from abalone gastropod muscle part II (PSCAGM-II) with yield of 8.7%. SDS-PAGE and amino acid composition indicated that the PSCAGM-II contained alpha(1) and alpha(2) chains and might be classified as type I collagen. PSCAGM-II exhibited a maximum absorbance at 232 nm, but little absorbance near to 280 nm. FTIR investigations showed the existence of helical arrangements of collagen. PSCAGM-II triple helical stereochemical structure was destroyed when the treated temperature up to 35 degrees C for 10 min. Denatured temperature detected by the viscometer was 22.9 degrees C when incubated for 20 min. Study on the properties and thermal stability of PSC from abalone gastropod muscle has certain directive significance for the abalone processing industry.