Yeast gene for a Tyr-DNA phosphodiesterase that repairs topoisomerase I complexes

被引：318

作者：

Pouliot, JJ ^{[1
]}

Yao, KC ^{[1
]}

Robertson, CA ^{[1
]}

Nash, HA ^{[1
]}

机构：

[1] NIMH, Mol Biol Lab, Bethesda, MD 20892 USA

来源：

SCIENCE | 1999年 / 286卷 / 5439期

关键词：

D O I：

10.1126/science.286.5439.552

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Covalent intermediates between topoisomerase I and DNA can become dead-end complexes that read to cell death. Here, the isolation of the gene for an enzyme that can hydrolyze the bond between this protein and DNA is described. Enzyme-defective mutants of yeast are hypersensitive to treatments that increase the amount of covalent complexes, indicative of enzyme involvement in repair. The gene is conserved in eukaryotes and identifies a family of enzymes that has not been previously recognized. The presence of this gene in humans may have implications for the effectiveness of topoisomerase I poisons, such as the camptothecins, in chemotherapy.

引用

页码：552 / 555

页数：4

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