Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

被引：50

作者：

Lee, Donghak ^{[1
]}

Ryu, Suho ^{[1
]}

Kim, Uihan ^{[1
]}

Jung, Daeseong ^{[1
]}

Joo, Chulmin ^{[1
]}

机构：

[1] Yonsei Univ, Dept Mech Engn, Seoul 120749, South Korea

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

SCANNING OPTICAL MICROSCOPY; ARRAY MICROSCOPE; LIGHT-MICROSCOPY; HIGH-RESOLUTION; SPLIT DETECTOR; ILLUMINATION; FIELD; APERTURE;

D O I：

10.1364/BOE.6.004912

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples. (C) 2015 Optical Society of America

引用

页码：4912 / 4922

页数：11

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