Extraction and electrophoretic analysis of large dsRNAs from desiccated plant tissues infected with plant viruses and biotrophic fungi

被引：24

作者：

Khankhum, S. ^{[1
]}

Escalante, C. ^{[1
]}

Rodrigues de Souto, E. ^{[2
]}

Valverde, R. A. ^{[1
]}

机构：

[1] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA

[2] Univ Estadual Maringa, Dept Agron, BR-87020900 Maringa, Parana, Brazil

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2017年 / 147卷 / 02期

基金：

美国食品与农业研究所;

关键词：

Biotrophic fungi; Large dsRNAs; Plant virus; Powderymildew; Rust; DOUBLE-STRANDED-RNA; TOBACCO-MOSAIC-VIRUS; MOLECULAR CHARACTERIZATION; RAPID ISOLATION; 1ST REPORT; DISEASE; CLONING; RUST; DNA; PARTICLES;

D O I：

10.1007/s10658-016-1014-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Extraction and electrophoretic analysis of large dsRNAs from plant and fungal tissues has been used successfully to detect RNA viruses infecting plants, fungi, and oomycetes. We modified a previously reported dsRNA extraction protocol and used it to detect a wide variety of plant and putative fungal viruses from infected plant tissues. The modified protocol was used successfully to extract large dsRNAs from 50 to 70 mg of desiccated plant tissues infected with acute and persistent RNA viruses and from plant tissues infected with biotrophic fungi causing rusts and powdery mildew diseases. The protocol proved to be efficient, fast, economic and versatile and requires relatively small amounts of desiccated tissue.

引用

页码：431 / 441

页数：11

共 51 条

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