Metabolic Pitfalls of CNS Cre-Based Technology

被引：163

作者：

Harno, Erika ^{[1
]}

Cottrell, Elizabeth C. ^{[2
]}

White, Anne ^{[1
,2
]}

机构：

[1] Univ Manchester, Fac Life Sci, Neurosci Res Grp, Manchester M13 9PT, Lancs, England

[2] Univ Manchester, Fac Med & Human Sci, Ctr Endocrinol & Diabet, Manchester M13 9PT, Lancs, England

来源：

CELL METABOLISM | 2013年 / 18卷 / 01期

关键词：

TRANSGENIC MOUSE LINE; NERVOUS-SYSTEM; MEDIATED RECOMBINATION; NEURONAL POPULATIONS; INSULIN-RESISTANCE; ADIPOSE-TISSUE; CELL-TYPES; GERM-LINE; IN-VIVO; GENE;

D O I：

10.1016/j.cmet.2013.05.019

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nestin-Cre mice have a significant metabolic phenotype that is hard to discern from current literature. Indeed, the Cre-lox system has numerous problems that can affect physiological parameters, and these are missed when the correct control strains are not used. Despite the increasing use of the Cre-lox system, these issues were not visible to the scientific community previously and may have affected published work. This makes it important to highlight the issues and raise awareness of the pitfalls of the Cre-lox system. Therefore, this perspective will discuss the impact of CNS and peripheral "off-target" Cre recombination on metabolic systems and describe the development of new approaches to obviate the difficulties.

引用

页码：21 / 28

页数：8

共 64 条

[11] The advent of AAV9 expands applications for brain and spinal cord gene delivery [J].

Dayton, Robert D. ;

Wang, David B. ;

Klein, Ronald L. .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2012, 12 (06) :757-766

[12] Nestin expression in pancreatic exocrine cell lineages [J].

Delacour, A ;

Nepote, V ;

Trumpp, A ;

Herrera, PL .

MECHANISMS OF DEVELOPMENT, 2004, 121 (01) :3-14

[13] A chemical-genetic approach for precise spatio-temporal control of cellular signaling [J].

Dong, Shuyun ;

Allen, John A. ;

Farrell, Martilias ;

Roth, Bryan L. .

MOLECULAR BIOSYSTEMS, 2010, 6 (08) :1376-1380

[14] Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/IoxP mediated recombination in the nervous system, kidney, and somite-derived tissues [J].

Dubois, Nicole C. ;

Hofmann, Denise ;

Kaloulis, Kostas ;

Bishop, J. M. ;

Trumpp, Andreas .

GENESIS, 2006, 44 (08) :355-360

[15] High levels of Cre expression in neuronal progenitors cause defects in brain development leading to microencephaly and hydrocephaly [J].

Forni, Paolo E. ;

Scuoppo, Claudio ;

Imayoshi, Itaru ;

Taulli, Riccardo ;

Dastru, Walter ;

Sala, Valentina ;

Betz, Ulrich A. K. ;

Muzzi, Patrizia ;

Martinuzzi, Daniela ;

Vercelli, Alessandro E. ;

Kageyama, Ryoichiro ;

Ponzetto, Carola .

JOURNAL OF NEUROSCIENCE, 2006, 26 (37) :9593-9602

[16] Nestin-Cre Mice Are Affected by Hypopituitarism, Which Is Not Due to Significant Activity of the Transgene in the Pituitary Gland [J].

Galichet, Christophe ;

Lovell-Badge, Robin ;

Rizzoti, Karine .

PLOS ONE, 2010, 5 (07)

[17]

Gannon M, 2000, GENESIS, V26, P143, DOI 10.1002/(SICI)1526-968X(200002)26:2<143::AID-GENE13>3.0.CO

[18]

2-L

[19] Disruption of neural signal transducer and activator of transcription 3 causes obesity, diabetes, infertility, and thermal dysregulation [J].

Gao, Q ;

Wolfgang, MJ ;

Neschen, S ;

Morino, K ;

Horvath, TL ;

Shulman, GI ;

Fu, XY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) :4661-4666

[20] GENETIC-TRANSFORMATION OF MOUSE EMBRYOS BY MICRO-INJECTION OF PURIFIED DNA [J].

GORDON, JW ;

SCANGOS, GA ;

PLOTKIN, DJ ;

BARBOSA, JA ;

RUDDLE, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7380-7384

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