Elevated Concentration of Microvesicles Isolated from Peripheral Blood in Breast Cancer Patients

被引：135
作者：
Galindo-Hernandez, Octavio [1 ]
Villegas-Comonfort, Socrates [1 ]
Candanedo, Fernando [1 ]
Gonzalez-Vazquez, Maria-Cristina [1 ]
Chavez-Ocana, Sonia [2 ]
Jimenez-Villanueva, Xicotencatl [3 ]
Sierra-Martinez, Monica [2 ]
Perez Salazar, Eduardo [1 ]
机构：
[1] IPN, CINVESTAV, Dept Biol Celular, Mexico City 07360, DF, Mexico
[2] Unidad Invest, Lab Genet & Diagnost Mol, Mexico City, DF, Mexico
[3] Hosp Juarez Mexico, Serv Oncol, Mexico City, DF, Mexico
来源：
ARCHIVES OF MEDICAL RESEARCH | 2013年 / 44卷 / 03期
关键词：
Breast cancer; Microvesicles; EGFR; FAK; FOCAL ADHESION KINASE; GROWTH-FACTOR RECEPTOR; TUMOR-DERIVED MICROVESICLES; PLATELET MICROPARTICLES; EXPRESSION; MEDIATORS; COMMUNICATION; PLAYERS; GENE; EGFR;
D O I：
10.1016/j.arcmed.2013.03.002
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background and Aims. Breast cancer is the most common cancer and the main cause of cancer deaths in women worldwide. Microvesicles (MVs) are fragments of the plasma membrane secreted from cytoplasmic membrane compartments by normal and malignant cells. An increase in MV number has been found in peripheral blood of patients with several diseases including cancer. We hypothesized that MV number and the relative amount of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) proteins in plasma fractions enriched in MVs and deprived of platelet-derived MVs are related to the presence of breast cancer. Methods. Plasma fractions enriched in MVs and deprived of platelet-derived MVs were obtained by differential centrifugation of blood samples. MV number was evaluated by BD TruCOUNT Tubes (BD Biosciences). FAK and EGFR proteins were analyzed by Western blot. Results. MV number in plasma fractions enriched with MVs and deprived of platelet-derived MVs is higher in breast cancer patients with stages I-IV as well as with T2-T4 tumors, in comparison to control group. In addition, plasma fractions enriched in MVs present FAK and EGFR proteins and their amount is increased in some stages of breast cancer in comparison to control group. Conclusions. Our findings strongly suggest that MV number and the amount of FAK and EGFR in plasma fractions enriched in MVs are associated with some stages of breast cancer. (C) 2013 IMSS. Published by Elsevier Inc.
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收藏
页码：208 / 214
页数：7
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