A Glucose/O2 Biofuel Cell Using Recombinant Thermophilic Enzymes

被引:0
作者
Wang, Xiuyun [1 ]
Li, Dean [1 ]
Watanabe, Tsuneaki [2 ]
Shigemori, Yasushi [2 ]
Mikawa, Tsutomu [3 ]
Okajima, Takeyoshi [1 ]
Mao, Lanqun [4 ]
Ohsaka, Takeo [1 ]
机构
[1] Tokyo Inst Technol, Dept Elect Chem, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan
[2] 2 1 5 Aisin Cosmos R&D Co Ltd, Kazusa Incubat Ctr, Chiba 2920818, Japan
[3] RIKEN, Spring Ctr 8, Sayo, Hyogo 6795148, Japan
[4] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
基金
日本科学技术振兴机构;
关键词
Biofuel cell; Thermophilic enzyme; Carbon nanotubes; Thermostability; Electrode modification; DEHYDROGENASE; PERFORMANCE; ELECTRODE; LACCASE; HB27;
D O I
暂无
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The utilization of recombinant thermophilic enzymes to construct a glucose/O-2 biofuel cell (BFC) workable in a wide temperature range with enhanced thermostability using thermophilic pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase (Tth-PQQGDH, active at even 100 degrees C) and laccase (Tth-Lcs) has been studied to improve the thermostability of BFC. In this glucose/O-2 biofuel cell system, electrodes were modified with single-walled carbon nanotubes (SWCNTs) based enzyme films. The resulting electrodes demonstrated enhanced biofuel-cell performance in wide temperature range, which allows them to be used at elevated temperatures.
引用
收藏
页码:1071 / 1078
页数:8
相关论文
共 27 条
[1]   Enzymatic biofuel cells for Implantable and microscale devices [J].
Barton, SC ;
Gallaway, J ;
Atanassov, P .
CHEMICAL REVIEWS, 2004, 104 (10) :4867-4886
[2]   Biofuel cells and their development [J].
Bullen, RA ;
Arnot, TC ;
Lakeman, JB ;
Walsh, FC .
BIOSENSORS & BIOELECTRONICS, 2006, 21 (11) :2015-2045
[3]   Enzyme catalysed biofuel cells [J].
Cooney, M. J. ;
Svoboda, V. ;
Lau, C. ;
Martin, G. ;
Minteer, S. D. .
ENERGY & ENVIRONMENTAL SCIENCE, 2008, 1 (03) :320-337
[4]   Biofuel cells - Recent advances and applications [J].
Davis, Frank ;
Higson, Seamus P. J. .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1224-1235
[5]   An enzymatic glucose/O2 biofuel cell:: Preparation, characterization and performance in serum [J].
Gao, Feng ;
Yan, Yiming ;
Su, Lei ;
Wang, Lun ;
Mao, Lanqun .
ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (05) :989-996
[6]   Miniature biofuel cells [J].
Heller, A .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2004, 6 (02) :209-216
[7]   MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE CRTB GENE OF THERMUS-THERMOPHILUS HB27, AN EXTREME THERMOPHILE PRODUCING CAROTENOID-PIGMENTS [J].
HOSHINO, T ;
FUJII, R ;
NAKAHARA, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (09) :3150-3153
[8]   Engineering PQQ glucose dehydrogenase with improved substrate specificity site-directed mutagenesis studies on the active center of PQQ glucose dehydrogenase [J].
Igarashi, S ;
Hirokawa, T ;
Sode, K .
BIOMOLECULAR ENGINEERING, 2004, 21 (02) :81-88
[9]   Bioelectrocatalysis-based application of quinoproteins and quinoprotein-containing bacterial cells in biosensors and biofuel cells [J].
Ikeda, T ;
Kano, K .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2003, 1647 (1-2) :121-126
[10]   A non-compartmentalized glucose|O2 biofuel cell by bioengineered electrode surfaces [J].
Katz, E ;
Willner, I ;
Kotlyar, AB .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 479 (01) :64-68