DETECTION AND GENOTYPING OF CLASSICAL SWINE FEVER VIRUS ISOLATES IN SERBIA

被引：2

作者：

Vesna, Milicevic ^{[1
]}

Sonja, Radojicic ^{[2
]}

Valcic, A. M. ^{[2
]}

Ivovic, V ^{[3
]}

Jelena, Maksimovic-Zoric ^{[1
]}

Radosavljevic, V ^{[2
]}

机构：

[1] Inst Vet Med Serbia, Belgrade, Serbia

[2] Univ Belgrade, Fac Vet Med, Belgrade, Serbia

[3] Ctr Parasit Zoonoses, Inst Med Res, Belgrade, Serbia

来源：

ACTA VETERINARIA-BEOGRAD | 2013年 / 63卷 / 2-3期

关键词：

Classical swine fever virus; genotyping; real time RT-PCR; Serbia; POLYMERASE-CHAIN-REACTION; RT-PCR; DISEASE; DISCRIMINATION; PESTIVIRUSES; STRAINS; ASSAY; PIGS;

D O I：

10.2298/AVB1303191M

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Classical swine fever (CSF) is a highly contagious disease of pigs leading to significant economic losses worldwide. Classical swine fever virus can be classified into three genogroups, each consisting of three or four subgroups. However, there is a lack of knowledge on the genotypes of CSFV isolates in Republic of Serbia. This study, based on the sequences analysis of partial E2 gene and 5' non coding region (NCR) of 15 CSFV isolated during 2006-2008 from domestic pigs, revealed that all were clustered into genetic group 2.3. Additionally, we showed that the two most often used real time RT-PCR assays were able to detect all local CSF viruses circulated in Serbia in the last years during intensive vaccination campaign against CSF

引用

页码：191 / 200

页数：10

共 30 条

[1]

[Anonymous], 2009, PRAVILNIK UTVRDIVANJ

[2]

[Anonymous], 2009, KLASICNA KUGA SVINJA, P251

[3] Highly sensitive one-tube RT-PCR and microplate hybridisation assay for the detection and for the discrimination of classical swine fever virus from other pestiviruses [J].