Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

被引：8

作者：

Schulte, Roberta ^{[1
]}

Talamas, Jessica ^{[1
]}

Doucet, Christine ^{[1
]}

Hetzer, Martin W. ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

来源：

PLOS ONE | 2008年 / 3卷 / 04期

关键词：

D O I：

10.1371/journal.pone.0002061

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.

引用

页数：6

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