Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

被引：8

作者：

Schulte, Roberta ^{[1
]}

Talamas, Jessica ^{[1
]}

Doucet, Christine ^{[1
]}

Hetzer, Martin W. ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

来源：

PLOS ONE | 2008年 / 3卷 / 04期

关键词：

D O I：

10.1371/journal.pone.0002061

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.

引用

页数：6

共 50 条

[21] SPINE:: A method for the rapid detection and analysis of protein-protein interactions in vivo [J].

Herzberg, Christina ;

Weidinger, Lope Andres Florez ;

Doerrbecker, Bastian ;

Huebner, Sebastian ;

Stuelke, Joerg ;

Commichau, Fabian M. .

PROTEOMICS, 2007, 7 (22) :4032-4035

[22] Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice [J].

Rohila, Jai S. ;

Chen, Mei ;

Chen, Shuo ;

Chen, Johann ;

Cerny, Ronald L. ;

Dardick, Christopher ;

Canlas, Patrick ;

Fujii, Hiroaki ;

Gribskov, Michael ;

Kanrar, Siddhartha ;

Knoflicek, Lucas ;

Stevenson, Becky ;

Xie, Mingtang ;

Xu, Xia ;

Zheng, Xianwu ;

Zhu, Jian-Kang ;

Ronald, Pamela ;

Fromm, Michael E. .

PLOS ONE, 2009, 4 (08)

[23] Detection of protein-protein interactions by protein fragment complementation strategies [J].

Michnick, SW ;

Remy, I ;

Campbell-Valois, FX ;

Vallée-Bélisle, A ;

Pelletier, JN .

APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PT C, 2000, 328 :208-230

[24] Detection of protein-protein interactions in vivo based on protein splicing [J].

Ozawa, T ;

Umezawa, Y .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (05) :578-583

[25] A protein complementation assay for detection of protein-protein interactions in vivo [J].

Pelletier, JN ;

Michnick, SW .

PROTEIN ENGINEERING, 1997, 10 :89-89

[26] Protein recruitment systems for the analysis of protein-protein interactions [J].

Aronheim, A .

BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) :1009-1013

[27] Mapping protein-protein interactions by affinity-directed mass spectrometry [J].

Zhao, YM ;

Muir, TW ;

Kent, SBH ;

Tischer, E ;

Scardina, JM ;

Chait, BT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4020-4024

[28] Deep neural networks for predicting the affinity landscape of protein-protein interactions [J].

Meiri, Reut ;

Lotati, Shay-Lee Aharoni ;

Orenstein, Yaron ;

Papo, Niv .

ISCIENCE, 2024, 27 (09)

[29] Following evolutionary paths to protein-protein interactions with high affinity and selectivity [J].

Levin, Kalia Bernath ;

Dym, Orly ;

Albeck, Shira ;

Magdassi, Shlomo ;

Keeble, Anthony H. ;

Kleanthous, Colin ;

Tawfik, Dan S. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2009, 16 (10) :1049-U67

[30] Following evolutionary paths to protein-protein interactions with high affinity and selectivity [J].

Kalia Bernath Levin ;

Orly Dym ;

Shira Albeck ;

Shlomo Magdassi ;

Anthony H Keeble ;

Colin Kleanthous ;

Dan S Tawfik .

Nature Structural & Molecular Biology, 2009, 16 :1049-1055

← 1 2 3 4 5 →