Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

被引：8
作者：
Schulte, Roberta [1 ]
Talamas, Jessica [1 ]
Doucet, Christine [1 ]
Hetzer, Martin W. [1 ]
机构：
[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA
来源：
PLOS ONE | 2008年 / 3卷 / 04期
关键词：
D O I：
10.1371/journal.pone.0002061
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] SPINE:: A method for the rapid detection and analysis of protein-protein interactions in vivo
    Herzberg, Christina
    Weidinger, Lope Andres Florez
    Doerrbecker, Bastian
    Huebner, Sebastian
    Stuelke, Joerg
    Commichau, Fabian M.
    PROTEOMICS, 2007, 7 (22) : 4032 - 4035
  • [22] Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice
    Rohila, Jai S.
    Chen, Mei
    Chen, Shuo
    Chen, Johann
    Cerny, Ronald L.
    Dardick, Christopher
    Canlas, Patrick
    Fujii, Hiroaki
    Gribskov, Michael
    Kanrar, Siddhartha
    Knoflicek, Lucas
    Stevenson, Becky
    Xie, Mingtang
    Xu, Xia
    Zheng, Xianwu
    Zhu, Jian-Kang
    Ronald, Pamela
    Fromm, Michael E.
    PLOS ONE, 2009, 4 (08):
  • [23] Detection of protein-protein interactions by protein fragment complementation strategies
    Michnick, SW
    Remy, I
    Campbell-Valois, FX
    Vallée-Bélisle, A
    Pelletier, JN
    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PT C, 2000, 328 : 208 - 230
  • [24] Detection of protein-protein interactions in vivo based on protein splicing
    Ozawa, T
    Umezawa, Y
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (05) : 578 - 583
  • [25] A protein complementation assay for detection of protein-protein interactions in vivo
    Pelletier, JN
    Michnick, SW
    PROTEIN ENGINEERING, 1997, 10 : 89 - 89
  • [26] Protein recruitment systems for the analysis of protein-protein interactions
    Aronheim, A
    BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) : 1009 - 1013
  • [27] Mapping protein-protein interactions by affinity-directed mass spectrometry
    Zhao, YM
    Muir, TW
    Kent, SBH
    Tischer, E
    Scardina, JM
    Chait, BT
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) : 4020 - 4024
  • [28] Deep neural networks for predicting the affinity landscape of protein-protein interactions
    Meiri, Reut
    Lotati, Shay-Lee Aharoni
    Orenstein, Yaron
    Papo, Niv
    ISCIENCE, 2024, 27 (09)
  • [29] Following evolutionary paths to protein-protein interactions with high affinity and selectivity
    Levin, Kalia Bernath
    Dym, Orly
    Albeck, Shira
    Magdassi, Shlomo
    Keeble, Anthony H.
    Kleanthous, Colin
    Tawfik, Dan S.
    NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2009, 16 (10) : 1049 - U67
  • [30] Following evolutionary paths to protein-protein interactions with high affinity and selectivity
    Kalia Bernath Levin
    Orly Dym
    Shira Albeck
    Shlomo Magdassi
    Anthony H Keeble
    Colin Kleanthous
    Dan S Tawfik
    Nature Structural & Molecular Biology, 2009, 16 : 1049 - 1055
12345