Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

被引：8
|
作者：
Schulte, Roberta [1 ]
Talamas, Jessica [1 ]
Doucet, Christine [1 ]
Hetzer, Martin W. [1 ]
机构：
[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA
来源：
PLOS ONE | 2008年 / 3卷 / 04期
关键词：
D O I：
10.1371/journal.pone.0002061
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
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页数：6
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