First clinical isolate of Escherichia coli harboring mcr-1 gene in Mexico

被引：19
作者：
Merida-Vieyra, Jocelin [1 ]
De Colsa-Ranero, Agustin [1 ,2 ]
Arzate-Barbosa, Patricia [3 ]
Arias-de la Garza, Eduardo [4 ]
Mendez-Tenorio, Alfonso [5 ]
Murcia-Garzon, Jazmin [5 ]
Aquino-Andrade, Alejandra [1 ]
机构：
[1] INP, Mol Microbiol Lab, Mexico City, DF, Mexico
[2] INP, Pediat Infect Dis Dept, Mexico City, DF, Mexico
[3] INP, Clin Bacteriol Lab, Mexico City, DF, Mexico
[4] INP, Healthcare Associated Infect Advisory Comm, Mexico City, DF, Mexico
[5] Inst Politecn Nacl, Natl Sch Biol Sci, Mexico City, DF, Mexico
来源：
PLOS ONE | 2019年 / 14卷 / 04期
关键词：
RESISTANCE GENE; BETA-LACTAMASES; MULTIPLEX PCR; SEQUENCE; ENTEROBACTERIACEAE; VIRULENCE; IDENTIFICATION; SURVEILLANCE; PLASMIDS; DATABASE;
D O I：
10.1371/journal.pone.0214648
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Our aim in this report was to describe the characteristics of the first clinical isolate of Escherichia coli (EC-PAG-733) harboring the mcr-1 gene found in Mexico. This isolate was obtained from a fecal sample from a young child with an oncological condition. We obtained the whole-genome sequence using next-generation sequencing and analyzed the sequence by bioinformatics tools. EC-PAG-733 was resistant to third- and fourth-generation cephalo-sporins and was susceptible to all carbapenems and amikacin; it was also resistant to cipro-floxacin, levofloxacin, gentamicin and colistin at a minimum inhibitory concentration (MIC) of 4 mu g/mL. This isolate was classified as O11:H25-ST457. EC-PAG-733 harbored an ESBL type CTX-M-55 as well as several virulence factors that have been associated with Enter-oaggregative Escherichia coli (EAEC). The mcr-1 gene was located within an Incl2 plasmid. The results of this whole genome shotgun project were deposited in DDBJ/ENA/GenBank under the accession number QKXE00000000.
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页数：11
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