Anti-metastatic effect of polysaccharide isolated from Colocasia esculenta is exerted through immunostimulation

被引:44
作者
Park, Hye-Ryung [1 ]
Lee, Hyun-Sun [2 ]
Cho, Sun Young [1 ]
Kim, Yoon-Sook [3 ]
Shin, Kwang-Soon [1 ]
机构
[1] Kyonggi Univ, Dept Food Sci & Biotechnol, Suwon 443760, Gyeonggi, South Korea
[2] Korea Univ, Dept Food & Nutr, Seoul 136703, South Korea
[3] Korea Food Res Inst, Songnam 463746, Gyeonggi, South Korea
关键词
anti-metastasis; Colocasia esculenta; acidic polysaccharide; natural killer cell; anti-complementary; MURAMYL DIPEPTIDE; CELLS; METASTASIS; COMPLEMENT; IMMUNITY; CANCER; MACROPHAGES; ACTIVATION; PATHWAY; PSK;
D O I
10.3892/ijmm.2012.1224
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
In the present study, an edible corm of the plant Colocasia esculenta, commonly known as Taro was extracted with cold water (4 degrees C). Finally, 10.44 g (1.04%) of the crude polysaccharide (Taro-0) was obtained from Taro. The purified active compound (Taro-4-I) was isolated using DEAE-Sepharose FF and Sephadex G-100. The anti-complementary activity of Taro-44 (57.3 +/- 4.5%) was similar to that of polysaccharide K (used as the positive control). The molecular weight of Taro-44 was 200 kDa and it was a polysaccharide composed of 64.4% neutral sugars and 35.6% uronic acid. Taro-44 activated the complement system through the classical and alternative pathways. The treatment of peritoneal macrophages with Taro-4-I significantly increased the production of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-alpha) in a dose-dependent manner. However, IL-12 production showed maximal activity at 56 mu g/ml and subsequently decreased. Splenocytes obtained from mice which were administered Taro-4-I intravenously showed a higher toxicity to Yac-1 cells compared to those obtained from untreated mice in a effector-to-target (E/T) ratio-dependent manner. The group treated with 50 mu g/ml Taro-44 showed a significantly increased toxicity to Yac-1 cells compared to the group treated with 500 mu g/ml Taro-4-I. The administration of Taro-4-I significantly inhibited the lung metastasis of B16BL6 melanoma cells. However, the group treated with 50 mu g/mouse Taro-4-I had a significantly lower number of tumors compared to the group injected with 500 mu g/mouse Taro-4-I.
引用
收藏
页码:361 / 368
页数:8
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