Intestinal Ralstonia pickettii augments glucose intolerance in obesity

被引：54

作者：

Udayappan, Shanthadevi D. ^{[1
]}

Kovatcheva-Datchary, Petia ^{[2
]}

Bakker, Guido J. ^{[1
]}

Havik, Stefan R. ^{[1
]}

Herrema, Hilde ^{[1
]}

Cani, Patrice D. ^{[3
]}

Bouter, Kristien E. ^{[1
]}

Belzer, Clara ^{[4
]}

Witjes, Julia J. ^{[1
]}

Vrieze, Anne ^{[5
]}

de Sonnaville, Noor ^{[5
]}

Chaplin, Alice ^{[1
]}

van Raalte, Daniel H. ^{[6
,7
]}

Aalvink, Steven ^{[4
]}

Dallinga-Thie, Geesje M. ^{[1
]}

Heilig, Hans G. H. J. ^{[4
]}

Bergstrom, Goran ^{[2
]}

van der Meij, Suzan ^{[8
]}

van Wagensveld, Bart A. ^{[9
]}

Hoekstra, Joost B. L. ^{[5
]}

Holleman, Frits ^{[5
]}

Stroes, Erik S. G. ^{[1
]}

Groen, Albert K. ^{[1
,10
]}

Backhed, Fredrik ^{[2
,11
]}

de Vos, Willem M. ^{[12
]}

Nieuwdorp, Max ^{[1
,2
,5
,6
,7
]}

机构：

[1] Acad Med Ctr, Dept Vasc Med, Amsterdam, Netherlands

[2] Univ Gothenburg, Sahlgrenska Univ Hosp, Wallenberg Lab, Gothenburg, Sweden

[3] Catholic Univ Louvain, WELBIO Walloon Excellence Life Sci & BIOtechnol, Louvain Drug Res Inst, Brussels, Belgium

[4] Wageningen Univ, Lab Microbiol, Wageningen, Netherlands

[5] Acad Med Ctr, Dept Internal Med, Amsterdam, Netherlands

[6] Vrije Univ Amsterdam, Med Ctr, Diabet Ctr, Dept Internal Med, Amsterdam, Netherlands

[7] Vrije Univ Amsterdam, Med Ctr, ICAR, Amsterdam, Netherlands

[8] Flevo Hosp, Dept Surg, Almere, Netherlands

[9] Sint Lucas Andreas Hosp, Dept Surg, Amsterdam, Netherlands

[10] Univ Groningen, Univ Med Ctr Groningen, Dept Pediat, Groningen, Netherlands

[11] Univ Copenhagen, Sect Metab Receptol & Enteroendocrinol, Novo Nordisk Fdn Ctr Basic Metab Res, Fac Hlth Sci, Copenhagen, Denmark

[12] Univ Helsinki, RPU Immunobiol, Helsinki, Finland

来源：

PLOS ONE | 2017年 / 12卷 / 11期

基金：

欧洲研究理事会; 瑞典研究理事会;

关键词：

LOW-GRADE INFLAMMATION; GUT MICROBIOTA; GASTROINTESTINAL-TRACT; INSULIN SENSITIVITY; METABOLIC SYNDROME; FAT; IMMUNITY; BACTERIA; TRANSLOCATION; INNATE;

D O I：

10.1371/journal.pone.0181693

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An altered intestinal microbiota composition has been implicated in the pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus (T2DM). Low grade inflammation, potentially initiated by the intestinal microbiota, has been suggested to be a driving force in the development of insulin resistance in obesity. Here, we report that bacterial DNA is present in mesenteric adipose tissue of obese but otherwise healthy human subjects. Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the Gram-negative species Ralstonia was most prevalent. Interestingly, fecal abundance of Ralstonia pickettii was increased in obese subjects with pre-diabetes and T2DM. To assess if R. pickettii was causally involved in development of obesity and T2DM, we performed a proof-of-concept study in diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. pickettii-treated DIO mice had reduced glucose tolerance. In addition, circulating levels of endotoxin were increased in R. pickettii-treated mice. In conclusion, this study suggests that intestinal Ralstonia is increased in obese human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.

引用

页数：15

共 43 条

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