Human erythrocyte acetylcholinesterase inhibition by cis-diamminediaquaplatinum (II):: a novel kinetic approach

The present work addresses the analyses of some novel kinetic parameters (k(t), K-v, t(50), K-ir, t(c), m(c), IC50, IC99 and K-i) of human erythrocyte membrane-bound acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by cis-diamminediaquaplatinum II (PDC). PDC is under a clinical trial for use as an antineoplastic drug. The authors recently reported that PDC and cisplatin have the ability to inhibit AChE activity in vitro. Therefore this study was designed to determine the estimation of time constant (k(t)), velocity constant (K-v), 50% inhibition time (t(50)), inhibition rate constant (K-ir), transition concentration (t(c)), meeting concentration (m(c)), 50% inhibition (IC50), 99% inhibition (IC99) and inhibition constant (K-i) by novel methods. The details are described in the text. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.