Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection

被引：50

作者：

Culha, M ^{[1
]}

Stokes, DL ^{[1
]}

Griffin, GD ^{[1
]}

Vo-Dinh, T ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Life Sci, Adv Biomed Sci & Technol Grp, Oak Ridge, TN 37831 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 09期

关键词：

biochip; molecular beacon; BRCA1; detection; fluorescence;

D O I：

10.1016/j.bios.2003.09.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is Successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70 nM. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1007 / 1012

页数：6

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