Using yeast RNA as a probe for generation of hydroxyl radicals by earth materials

被引:38
作者
Cohn, CA [1 ]
Laffers, R
Schoonen, MAA
机构
[1] SUNY Stony Brook, Dept Geosci, Stony Brook, NY 11794 USA
[2] SUNY Stony Brook, Ctr Environm Mol Sci, Stony Brook, NY 11794 USA
关键词
D O I
10.1021/es052301k
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Inhalation of certain types of particulate matter can lead to lung disease. The reactivity of these particles and, in part, the pathologic responses that result are dictated by their physicochemical properties. The ability of particles to induce the generation of reactive oxygen species (ROS), especially hydroxyl radicals in vivo, is one property that has been correlated to the development of lung disease. Several minerals, such as quartz and asbestos, are known to generate hydroxyl radicals and cause lung disease, but many other minerals have never been tested. Here, we describe a technique employing yeast RNA as a probe to screen for mineral-generated hydroxyl radicals. The stability of RNA in the presence of hydrogen peroxide, ferrous iron, hydroxyl radicals, and several common minerals (quartz, albite, forsterite, fayalite, hematite, magnetite, coal, and pyrite) was examined. 3'-(p-Aminophenyl) fluorescein (APF) was used to verify mineral generation of ROS. RNA is stable in the presence of hydrogen peroxide, quartz, and albite; while it degrades in the presence of ferrous iron, hydroxyl radicals, and the other minerals. Coal and pyrite are the most reactive both in RNA degradation and hydroxyl radical generation. This noncellular technique provides a straightforward way to compare many different particles simultaneously. Those particles showing reactivity toward RNA using this method are high-priority candidates for further in vitro and possibly in vivo tests.
引用
收藏
页码:2838 / 2843
页数:6
相关论文
共 58 条
[1]   Inflammatory time course after quartz instillation -: Role of tumor necrosis factor-α and particle surface [J].
Albrecht, C ;
Schins, RPF ;
Höhr, D ;
Becker, A ;
Shi, TM ;
Knaapen, AM ;
Borm, PJA .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2004, 31 (03) :292-301
[2]  
[Anonymous], 1997, IARC MON EV CARC RIS, V70
[3]   Phagocytes and oxidative stress [J].
Babior, BM .
AMERICAN JOURNAL OF MEDICINE, 2000, 109 (01) :33-44
[4]   Rat lung inflammatory responses after in vivo and in vitro exposure to various stone particles [J].
Becher, R ;
Hetland, RB ;
Refsnes, M ;
Dahl, JE ;
Dahlman, HJ ;
Schwarze, PE .
INHALATION TOXICOLOGY, 2001, 13 (09) :789-805
[5]   RADICAL OXIDATION REACTIONS OF THE PURINE MOIETY OF 2'-DEOXYRIBONUCLEOSIDES AND DNA BY IRON-CONTAINING MINERALS [J].
BERGER, M ;
DEHAZEN, M ;
NEJJARI, A ;
FOURNIER, J ;
GUIGNARD, J ;
PEZERAT, H ;
CADET, J .
CARCINOGENESIS, 1993, 14 (01) :41-46
[6]   Pyrite-Induced Hydrogen Peroxide Formation as a Driving Force in the Evolution of Photosynthetic Organisms on an Early Earth [J].
Borda, Michael J. ;
Elsetinow, Alicia R. ;
Schoonen, Martin A. ;
Strongin, Daniel R. .
ASTROBIOLOGY, 2001, 1 (03) :283-288
[7]   REACTIONS OF OXYL RADICALS WITH DNA [J].
BREEN, AP ;
MURPHY, JA .
FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (06) :1033-1077
[8]   Mineralogy of agricultural source soil and respirable dust in California [J].
Clausnitzer, H ;
Singer, MJ .
JOURNAL OF ENVIRONMENTAL QUALITY, 1999, 28 (05) :1619-1629
[9]   Coal mining and chronic obstructive pulmonary disease: a review of the evidence [J].
Coggon, D ;
Taylor, AN .
THORAX, 1998, 53 (05) :398-407
[10]  
COHEN CA, 2006, IN PRESS GEOCHEM T