Tracking the Dynamic Folding and Unfolding of RNA G-Quadruplexes in Live Cells

被引：136

作者：

Chen, Xiu-Cai ^{[1
]}

Chen, Shuo-Bin ^{[1
]}

Dai, Jing ^{[1
]}

Yuan, Jia-Hao ^{[1
]}

Ou, Tian-Miao ^{[1
]}

Huang, Zhi-Shu ^{[1
]}

Tan, Jia-Heng ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 17期

基金：

中国国家自然科学基金;

关键词：

fluorescent probes; folding and unfolding; G-quadruplexes; live-cell imaging; RNA structures; MESSENGER-RNA; SHAPE REVEALS; LIVING CELLS; VISUALIZATION; TRANSLATION; TELOMERES; HELICASE; INSIGHTS; BIOLOGY; 5'-UTR;

D O I：

10.1002/anie.201801999

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Because of the absence of methods for tracking RNA G-quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G-quadruplexes is so far limited. Herein, we report a new red-emitting fluorescent probe, QUMA-1, for the selective, continuous, and real-time visualization of RNA G-quadruplexes in live cells. The applications of QUMA-1 in several previously intractable applications, including live-cell imaging of the dynamic folding, unfolding, and movement of RNA G-quadruplexes and the visualization of the unwinding of RNA G-quadruplexes by RNA helicase have been demonstrated. Notably, our real-time results revealed the complexity of the dynamics of RNA G-quadruplexes in live cells. We anticipate that the further application of QUMA-1 in combination with appropriate biological and imaging methods to explore the dynamics of RNA G-quadruplexes will uncover more information about the biological roles of RNA G-quadruplexes.

引用

页码：4702 / 4706

页数：5

共 45 条

[1]

[Anonymous], 2016, ANGEW CHEM, V128, P3279

[2] Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy [J].

Bao, Hong-Liang ;

Ishizuka, Takumi ;

Sakamoto, Takashi ;

Fujimoto, Kenzo ;

Uechi, Tamayo ;

Kenmochi, Naoya ;

Xu, Yan .

NUCLEIC ACIDS RESEARCH, 2017, 45 (09) :5501-5511

[3]

Biffi G, 2014, NAT CHEM, V6, P75, DOI [10.1038/NCHEM.1805, 10.1038/nchem.1805]

[4] Biophysical characterization of G-quadruplex forming FMR1 mRNA and of its interactions with different fragile X mental retardation protein isoforms [J].

Blice-Baum, Anna C. ;

Mihailescu, Mihaela-Rita .

RNA, 2014, 20 (01) :103-114

[5] Structural basis of transcription inhibition by α-amanitin and implications for RNA polymerase II translocation [J].

Brueckner, Florian ;

Cramer, Patrick .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2008, 15 (08) :811-818

[6] 5′-UTR RNA G-quadruplexes: translation regulation and targeting [J].

Bugaut, Anthony ;

Balasubramanian, Shankar .

NUCLEIC ACIDS RESEARCH, 2012, 40 (11) :4727-4741

[7] The G-quadruplex-interactive molecule BRACO-19 inhibits tumor growth, consistent with telomere targeting and interference with telomerase function [J].

Burger, AM ;

Dai, FP ;

Schultes, CM ;

Reszka, AP ;

Moore, MJ ;

Double, JA ;

Neidle, S .

CANCER RESEARCH, 2005, 65 (04) :1489-1496

[8] RNA G-quadruplexes: emerging mechanisms in disease [J].

Cammas, Anne ;

Millevoi, Stefania .

NUCLEIC ACIDS RESEARCH, 2017, 45 (04) :1584-1595

[9] Insights into the mechanism of a G-quadruplex-unwinding DEAH-box helicase [J].

Chen, Michael C. ;

Murat, Pierre ;

Abecassis, Keren ;

Ferre-D'Amare, Adrian R. ;

Balasubramanian, Shankar .

NUCLEIC ACIDS RESEARCH, 2015, 43 (04) :2223-2231

[10] Visualization of NRAS RNA G-Quadruplex Structures in Cells with an Engineered Fluorogenic Hybridization Probe [J].

Chen, Shuo-Bin ;

Hu, Ming-Hao ;

Liu, Guo-Cai ;

Wang, Jin ;

Ou, Tian-Miao ;

Gu, Lian-Quan ;

Huang, Zhi-Shu ;

Tan, Jia-Heng .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2016, 138 (33) :10382-10385

← 1 2 3 4 5 →