Reevaluation of a lectin antibody ELISA kit for measuring fucosylated haptoglobin in various conditions

被引：34

作者：

Kamada, Yoshihiro ^{[1
]}

Kinoshita, Noriaki ^{[2
]}

Tsuchiya, Yoko ^{[2
]}

Kobayashi, Kyoko ^{[2
]}

Fujii, Hironobu ^{[1
]}

Terao, Naoko ^{[1
]}

Kamihagi, Kyoko ^{[3
]}

Koyama, Nobuto ^{[3
]}

Yamada, Shin ^{[4
]}

Daigo, Yataro ^{[5
,6
,7
]}

Nakamura, Yusuke ^{[7
]}

Taniguchi, Naoyuki ^{[8
]}

Miyoshi, Eiji ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Mol Biochem & Clin Invest, Suita, Osaka 5650871, Japan

[2] Immunobiol Labs Co Ltd, Gunma 3750005, Japan

[3] Takara Bio Inc, Otsu, Shiga 5202193, Japan

[4] AMs New Otani Clin, Osaka 5400001, Japan

[5] Shiga Univ Med Sci, Dept Med Oncol, Otsu, Shiga 5202192, Japan

[6] Shiga Univ Med Sci, Ctr Canc, Otsu, Shiga 5202192, Japan

[7] Univ Tokyo, Mol Med Lab, Ctr Human Genome, Inst Med Sci,Minato Ku, Tokyo 1088639, Japan

[8] RIKEN, Adv Sci Inst, Syst Glycobiol Res Grp, Dis Glyc Team, Wako, Saitama 3510198, Japan

来源：

CLINICA CHIMICA ACTA | 2013年 / 417卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Fucosylation; Haptoglobin; Lectin ELISA; Oligosaccharide; PANCREATIC-CANCER; ALPHA-FETOPROTEIN; MARKER; GLYCOSYLATION; SERA;

D O I：

10.1016/j.cca.2012.12.014

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Fucosylated haptoglobin (Fuc-Hpt) is a novel cancer biomarker in a variety of pathological conditions. We previously found that the level of Fuc-Hpt is increased in the sera of patients with pancreatic cancer, and established a lectin antibody ELISA using Aleuria aurantia lectin, which specifically binds to fucosylated residues on oligosaccharides. Methods: To apply this assay system to the clinical detection of several diseases, several assay conditions such as serum dilutions and inhibitory factors were investigated. The Fuc-Hpt kit was available for 25-625 fold serum dilution. Results: While the values of Fuc-Hpt assay using sera and plasma were different, they showed positive correlation. The addition of bilirubin and formagine did not influence on Fuc-Hpt assay, but hemoglobin inhibited this assay in a dose-dependent manner. Conclusions: We reevaluated this lectin antibody ELISA kit for measuring fucosylated haptoglobin in various conditions in this study. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：48 / 53

页数：6

共 14 条

[1] CARBOHYDRATE-BASED MEASUREMENTS ON ALPHA-FETOPROTEIN IN THE EARLY DIAGNOSIS OF HEPATOCELLULAR-CARCINOMA [J].