Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

被引：67

作者：

Mancini, Nicasio ^{[1
]}

De Carolis, Elena ^{[2
]}

Infurnari, Laura ^{[1
]}

Vella, Antonietta ^{[2
]}

Clementi, Nicola ^{[1
]}

Vaccaro, Luisa ^{[2
]}

Ruggeri, Alberto ^{[2
]}

Posteraro, Brunella ^{[3
]}

Burioni, Roberto ^{[1
]}

Clementi, Massimo ^{[1
]}

Sanguinetti, Maurizio ^{[2
]}

机构：

[1] Univ Vita Salute San Raffaele, Lab Microbiol & Virol, Milan, Italy

[2] Univ Cattolica Sacro Cuore, Ist Microbiol, Rome, Italy

[3] Univ Cattolica Sacro Cuore, Ist Igiene, Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 07期

关键词：

MICROBIOLOGY LABORATORIES; ROUTINE IDENTIFICATION; CANDIDA; DISCRIMINATION; BACTERIA;

D O I：

10.1128/JCM.00841-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P<0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).

引用

页码：2453 / 2457

页数：5

共 22 条

[1] Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [J].

Bader, O. ;

Weig, M. ;

Taverne-Ghadwal, L. ;

Lugert, R. ;

Gross, U. ;

Kuhns, M. .

CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (09) :1359-1365

[2] Comparison of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis adhesive properties and pathogenicity [J].

Bertini, Alessia ;

De Bernardis, Flavia ;

Hensgens, Lambert A. M. ;

Sandini, Silvia ;

Senesi, Sonia ;

Tavanti, Arianna .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 303 (02) :98-103

[3] Direct Bacterial Identification in Positive Blood Cultures by Use of Two Commercial Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems [J].

Chen, Jonathan H. K. ;

Ho, Pak-Leung ;

Kwan, Grace S. W. ;

She, Kevin K. K. ;

Siu, Gilman K. H. ;

Cheng, Vincent C. C. ;

Yuen, Kwok-Yung ;

Yam, Wing-Cheong .

JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (06) :1733-1739

[4] Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Caspofungin Susceptibility Testing of Candida and Aspergillus Species [J].

De Carolis, Elena ;

Vella, Antonietta ;

Florio, Ada R. ;

Posteraro, Patrizia ;

Perlin, David S. ;

Sanguinetti, Maurizio ;

Posteraro, Brunella .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (07) :2479-2483

[5] Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Routine Identification of Yeast [J].

Dhiman, Neelam ;

Hall, Leslie ;

Wohlfiel, Sherri L. ;

Buckwalter, Seanne P. ;

Wengenack, Nancy L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1614-1616

[6] Evaluation of species-specific PCR, Bruker MS, VITEK MS and the VITEK 2 system for the identification of clinical Enterococcus isolates [J].

Fang, H. ;

Ohlsson, A. -K. ;

Ullberg, M. ;

Ozenci, V. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (11) :3073-3077

[7] Real-Time Identification of Bacteria and Candida Species in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].

Ferroni, Agnes ;

Suarez, Stephanie ;

Beretti, Jean-Luc ;

Dauphin, Brunhilde ;

Bille, Emmanuelle ;

Meyer, Julie ;

Bougnoux, Marie-Elisabeth ;

Alanio, Alexandre ;

Berche, Patrick ;

Nassif, Xavier .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (05) :1542-1548

[8] MALDI-TOF MS Enables the Rapid Identification of the Major Molecular Types within the Cryptococcus neoformans/C. gattii Species Complex [J].

Firacative, Carolina ;

Trilles, Luciana ;

Meyer, Wieland .

PLOS ONE, 2012, 7 (05)

[9] Optimization of the Preanalytical Steps of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Identification Provides a Flexible and Efficient Tool for Identification of Clinical Yeast Isolates in Medical Laboratories [J].

Goyer, Marianne ;

Lucchi, Geraldine ;

Ducoroy, Patrick ;

Vagner, Odile ;

Bonnin, Alain ;

Dalle, Frederic .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (09) :3066-3068

[10] Routine Identification of Medical Fungi by the New Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight System with a New Time-Effective Strategy [J].

Iriart, Xavier ;

Lavergne, Rose-Anne ;

Fillaux, Judith ;

Valentin, Alexis ;

Magnaval, Jean-Francois ;

Berry, Antoine ;

Cassaing, Sophie .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (06) :2107-2110

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