DNA-Mediated Assembly of Protein Heterodimers on Membrane Surfaces

被引：24

作者：

Coyle, Michael P. ^{[1
,2
,3
,4
]}

Xu, Qian ^{[1
,2
,3
,4
]}

Chiang, Samantha ^{[1
]}

Francis, Matthew B. ^{[1
,3
,4
]}

Groves, Jay T. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Mat Sci, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 13期

基金：

美国国家卫生研究院;

关键词：

EPH RECEPTORS; IMMUNOLOGICAL SYNAPSE; SIGNALING CLUSTERS; CELL-ADHESION; EPHRINS; ANTIGEN; ORGANIZATION; ACTIVATION; ANTIBODIES; KINETICS;

D O I：

10.1021/ja3101215

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a method based on self-assembling oligonucleotides to anchor proteins to a supported membrane surface. This anchoring method allows control of the surface density of multiple proteins. By incorporating additional recognition sequences into the DNA linkers, defined heterodimers can be produced upon the addition of a heterospecific DNA cross-linking strand. Characterization by fluorescence cross-correlation spectroscopy (FCCS) confirmed lateral mobility and the formation of specific heterodimers. We further demonstrate that proteins linked in this manner as either monomers or dimers can form functional interfaces with living cells.

引用

页码：5012 / 5016

页数：5

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