ABPP Methodology: Introduction and Overview

被引：29
作者：
Nodwell, Matthew B. [1 ]
Sieber, Stephan A. [1 ]
机构：
[1] Tech Univ Munich, Dept Chem, Ctr Integrated Prot Sci CIPSM, Inst Adv Studies, D-85747 Garching, Germany
来源：
ACTIVITY-BASED PROTEIN PROFILING | 2012年 / 324卷
基金：
欧洲研究理事会;
关键词：
Activity-based protein profiling; Analytical chemistry; Chemical biology; Proteomics; ACTIVITY-BASED PROBES; PROTEIN LYSINE METHYLTRANSFERASE; SERINE HYDROLASE ACTIVITIES; ENZYME-ACTIVITY PROFILES; ARGININE DEIMINASE 4; CYSTEINE PROTEASE; CATHEPSIN-B; HISTONE METHYLTRANSFERASE; KINASE INHIBITORS; IN-VITRO;
D O I：
10.1007/128_2011_302
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Activity-based protein profiling (ABPP) is emerging as a mature method for chemically interrogating the proteome of a cell. This chapter serves to introduce the reader to ABPP by providing overviews of the general principles of the technique, analytical methods used in ABPP, the classes of enzymes that can be specifically addressed by ABPP probes, and biological applications of ABPP.
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收藏
页码：1 / 41
页数：41
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