Preparative chromatographic resolution of racemates using HPLC and SFC in a pharmaceutical discovery environment

被引：84

作者：

Miller, Larry ^{[1
]}

Potter, Matt ^{[1
]}

机构：

[1] Amgen Inc, Cambridge, MA 02139 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 01期

关键词：

Chiral separations; Preparative chromatography; Supercritical fluid chromatography; Chiral stationary phase;

D O I：

10.1016/j.jchromb.2008.06.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparative chromatographic resolution of racemates has become a standard approach for the generation of enantiomers in pharmaceutical discovery laboratories. This paper will discuss the use of preparative HPLC and SFC to generate individual enantiomers for discovery activities. Analytical HPLC and SFC method development to rapidly screen chiral stationary phases and solvent combinations will be presented. The usefulness of preparative chromatographic resolution of racemates will be demonstrated through the presentation of numerous non-routine case studies from the laboratories at Amgen. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：230 / 236

页数：7

共 27 条

[1] Use of achiral/chiral SFC/MS for the profiling of isomeric cinnamonitrile/hydrocinnamonitrile products in chiral drug synthesis [J].