Effects of Cosolvents on the Folding and Catalytic Activities of the Hammerhead Ribozyme

被引：23

作者：

Nakano, Shu-ichi ^{[1
]}

Kitagawa, Yuichi ^{[1
]}

Yamashita, Hirofumi ^{[1
]}

Miyoshi, Daisuke ^{[1
]}

Sugimoto, Naoki ^{[1
,2
]}

机构：

[1] Konan Univ, Fac Frontiers Innovat Res Sci & Technol FIRST, Dept Nanobiochem, Chuo Ku, Kobe, Hyogo 6500047, Japan

[2] Konan Univ, Frontier Inst Biomol Engn Res, Chuo Ku, Kobe, Hyogo 6500047, Japan

来源：

CHEMBIOCHEM | 2015年 / 16卷 / 12期

关键词：

FRET; kinetics; melting temperatures; ribozymes; RNA base-pairs; solvent effects; DIELECTRIC-PROPERTIES; OSMOTIC-PRESSURE; DNA; HAIRPIN; ION; MONOVALENT; HYDRATION; BIOSENSOR; MECHANISM; MAGNESIUM;

D O I：

10.1002/cbic.201500139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNA cleavage activity of the hammerhead ribozyme has been compared in various mixed aqueous solutions containing cosolvents. Kinetic analysis revealed that the tested cosolvents enhanced the ribozyme activity, particularly at low MgCl2 concentrations. These enhancements, in some cases of more than tenfold, resulted from a reduction in the Mg2+ concentration required for substrate cleavage. An inverse correlation was found between the MgCl2 concentration essential for efficient catalysis and the dielectric constant values. In contrast, FRET measurements showed no substantial influence of cosolvents on the Mg2+-induced structural transitions. The results suggest that the solution environment has various effects on the Mg2+ interactions involved in the catalysis and global folding of the ribozyme.

引用

页码：1803 / 1810

页数：8

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