Effect of the 67Zn isotope on leukemic cells and normal lymphocytes

被引：9
作者：
Orlova, M. A. [1 ]
Osipova, E. Yu. [2 ]
Rumyantsev, S. A. [2 ]
Ashurko, S. P. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia
[2] Fed Sci & Clin Ctr Pediat Hematol Immunol & Oncol, Moscow 119991, Russia
来源：
RUSSIAN CHEMICAL BULLETIN | 2012年 / 61卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
fullerene derivatives; isotopy; Zn-67 and Mg-25 isotopes; acute leukemia; nanoparticles; cytofluorimetry; apoptosis; MTT test; ASSAYS;
D O I：
10.1007/s11172-012-0057-y
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Porphyrinofullerene nanoparticles (NP) containing magnetic isotopes Mg-25 and Zn-67 (Mg-25-NP and Zn-67-NP) and the natural isotopic composition of zinc (Zn) were tested on human leukemic cells of patients with acute leukemia and on lymphocytes of healthy donors. The fundamental differences in the cytotoxic effect of magnetic and nonmagnetic zinc isotopes on tumor cells were observed, as well as the complete absence of the influence of the magnetic magnesium isotope and pristine nanoparticles. The Zn-67-NP manifested high cytotoxicity towards cells of acute B-lymphoblast leukemia with LD50 almost three times lower than that of healthy donors and four times lower than that of the Zn-NP. Apoptosis was evaluated by cytofluorimetry for the drugs used.
引用
收藏
页码：405 / 408
页数：4
相关论文
共 11 条
  • [1] [Anonymous], Eur. Pat., Patent No. [EP 1992627 A1, 1992627]
  • [2] Buchachenko A. L., 2010, Int. J. Mol. Med. Adv. Sci, V6, P34, DOI [10.3923/ijmmas.2010.34.37, DOI 10.3923/IJMMAS.2010.34.37]
  • [3] Magnesium isotope effects in enzymatic phosphorylation
    Buchachenko, Anatoly L.
    Kouznetsov, Dmitry A.
    Breslavskaya, Natalia N.
    Orlova, Marina A.
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (08) : 2548 - 2556
  • [4] Cytosolic zinc buffering and muffling: Their role in intracellular zinc homeostasis
    Colvin, Robert A.
    Holmes, William R.
    Fontaine, Charles P.
    Maret, Wolfgang
    [J]. METALLOMICS, 2010, 2 (05) : 306 - 317
  • [5] Intracellular zinc increase inhibits p53-/- pancreatic adenocarcinoma cell growth by ROS/AIF-mediated apoptosis
    Donadelli, M.
    Pozza, E. Dalla
    Scupoli, M. T.
    Costanzo, C.
    Scarpa, A.
    Palmieri, M.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2009, 1793 (02): : 273 - 280
  • [6] Kaspers G. J. L., 1993, DRUG RESISTANCE LEUK, P329
  • [7] Denaturational Stress Induces Formation of Zinc-Deficient Monomers of Cu,Zn Superoxide Dismutase: Implications for Pathogenesis in Amyotrophic Lateral Sclerosis
    Mulligan, Vikram Khipple
    Kerman, Aaron
    Ho, Sylvia
    Chakrabartty, Avijit
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2008, 383 (02) : 424 - 436
  • [8] Ormerod M.G., 1994, FLOW CYTOMETRY PRACT, P119
  • [9] The porphyrin-fullerene nanoparticles to promote the ATP overproduction in myocardium: 25Mg2+-magnetic isotope effect
    Rezayat, S. M.
    Boushehri, S. V. S.
    Salmanian, B.
    Omidvari, A. H.
    Tarighat, S.
    Esmaeili, S.
    Sarkar, S.
    Amirshahi, N.
    Alyautdin, R. N.
    Orlova, M. A.
    Trushkov, I. V.
    Buchachenko, A. L.
    Liu, K. C.
    Kuznetsov, D. A.
    [J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2009, 44 (04) : 1554 - 1569
  • [10] DRUG SENSITIVITY ASSAYS IN LEUKEMIA AND LYMPHOMA
    VEERMAN, AJP
    PIETERS, R
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 1990, 74 (04) : 381 - 384
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