Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi

被引：15

作者：

Yang, Feng Li ^{[1
]}

Li, Xiao Shan ^{[1
]}

Liang, Xian Wei ^{[1
]}

Zhang, Xiu Fang ^{[1
]}

Qin, Guang Sheng ^{[1
]}

Yang, Bing Zhuang ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Guangxi Key Lab Buffalo Genet & Breeding, Guangxi Buffalo Res Inst, Nanning, Peoples R China

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2012年 / 44卷 / 08期

关键词：

Bovine mastitis; Staphylococcus aureus; Virulence-associated genes; Polymerase chain reaction (PCR); POLYMERASE-CHAIN-REACTION; SUPERANTIGENIC TOXIN GENES; PCR-BASED DETECTION; SUBCLINICAL MASTITIS; NUCLEOTIDE-SEQUENCE; BETA-HEMOLYSIN; DAIRY FARMS; IDENTIFICATION; PREVALENCE; ASSAY;

D O I：

10.1007/s11250-012-0143-z

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals and is one of the most common etiological agents of clinical and subclinical bovine mastitis. The purpose of this study was to determine the presence of genes encoding clfA, fnbA, fnbB, cap5, cap8, hla, hlb, nuc, sea, and tst of S. aureus strains (n = 39) isolated from bovine clinical mastitis in Guangxi by polymerase chain reaction amplification. The results of the present study indicated that all isolates were found to contain one or more virulence-associated genes. The most frequently encountered genes were fnbA (97 %) and nuc (90 %), followed by hla (85 %) and hlb (82 %), respectively. None of the investigated S. aureus strains harbored fnbB and sea genes. The data in the present study showed a relatively wide distribution of the genes fnbA and nuc among the investigated isolates, indicating that they play an important role on bovine mastitis pathogenesis. The study provides a valuable insight into the virulence-associated genes of this important pathogen.

引用

页码：1821 / 1826

页数：6

共 52 条

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