Functional analysis of proteins involved in movement of the monopartite begomovirus, tomato yellow leaf curl virus

被引:245
作者
Rojas, MR
Jiang, H
Salati, R
Xoconostle-Cázares, B
Sudarshana, MR
Lucas, WJ
Gilbertson, RL [1 ]
机构
[1] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
[2] Univ Calif Davis, Plant Biol Sect, Div Biol Sci, Davis, CA 95616 USA
基金
美国国家科学基金会;
关键词
begomovirus; capsid protein; geminivirus; green fluorescent protein; immunolocalization; microinjection; movement protein; plant virus movement; phloem-limited virus; plasmodesmata; TYLCV;
D O I
10.1006/viro.2001.1194
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The functional properties of proteins [capsid protein (CP), V1, and C4] potentially involved with movement of the monopartite begomovirus, Tomato yellow leaf curl virus (TYLCV), were investigated using microinjection of Escherichia coli expressed proteins and transient expression of GFP fusion proteins. The TYLCV CP localized to the nucleus and nucleolus and acted as a nuclear shuttle, facilitating import and export of DNA. Thus, the CP serves as the functional homolog of the bipartite begomovirus BV1. The TYLCV V1 localized around the nucleus and at the cell periphery and colocalized with the endoplasmic reticulum, whereas C4 was localized to the cell periphery. Together, these patterns of localization were similar to that of the bipartite begomovirus BC1, known to mediate cell-to-cell movement However, in contrast to BC1, V1 and C4, alone or in combination, had a limited capacity to move and mediate macromolecular trafficking through mesophyll or epidermal plasmodesmata. Immunolocalization and in situ PCR experiments, conducted with tomato plants at three stages of development, established that TYLCV infection was limited to phloem cells of shoot apical, leaf, stem, and floral tissues. Thus, the V1 and/or C4 may be analogs of the bipartite begomovirus BC1 that have evolved to mediate TYLCV movement within phloem tissue. (C) 2001 Elsevier Science.
引用
收藏
页码:110 / 125
页数:16
相关论文
共 64 条
  • [1] [Anonymous], CAN J BOT
  • [2] WHITEFLY TRANSMISSION AND EFFICIENT SSDNA ACCUMULATION OF BEAN GOLDEN MOSAIC GEMINIVIRUS REQUIRE FUNCTIONAL COAT PROTEIN
    AZZAM, O
    FRAZER, J
    DELAROSA, D
    BEAVER, JS
    AHLQUIST, P
    MAXWELL, DP
    [J]. VIROLOGY, 1994, 204 (01) : 289 - 296
  • [3] EFFECTS OF MUTAGENESIS INVITRO ON THE ABILITY OF CLONED TOMATO GOLDEN MOSAIC-VIRUS DNA TO INFECT NICOTIANA-BENTHAMIANA PLANTS
    BROUGH, CL
    HAYES, RJ
    MORGAN, AJ
    COUTTS, RHA
    BUCK, KW
    [J]. JOURNAL OF GENERAL VIROLOGY, 1988, 69 : 503 - 514
  • [4] Carrington JC, 1996, PLANT CELL, V8, P1669, DOI 10.1105/tpc.8.10.1669
  • [5] CHERIF C, 1983, PHYTOPATHOL Z, V108, P221
  • [6] Cohen S., 1994, Advances in Disease Vector Research, V10, P259
  • [7] Phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (BC1) proteins
    Duan, YP
    Powell, CA
    Purcifull, DE
    Broglio, P
    Hiebert, E
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (09) : 1065 - 1074
  • [8] DELIMITATION OF ESSENTIAL GENES OF CASSAVA LATENT VIRUS DNA-2
    ETESSAMI, P
    CALLIS, R
    ELLWOOD, S
    STANLEY, J
    [J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (11) : 4811 - 4829
  • [9] GENETIC-ANALYSIS OF TOMATO GOLDEN MOSAIC-VIRUS - THE COAT PROTEIN IS NOT REQUIRED FOR SYSTEMIC SPREAD OR SYMPTOM DEVELOPMENT
    GARDINER, WE
    SUNTER, G
    BRAND, L
    ELMER, JS
    ROGERS, SG
    BISARO, DM
    [J]. EMBO JOURNAL, 1988, 7 (04) : 899 - 904
  • [10] Ghoshroy Soumitra, 1997, Annu Rev Plant Physiol Plant Mol Biol, V48, P27, DOI 10.1146/annurev.arplant.48.1.27