In planta particle bombardment (iPB): A new method for plant transformation and genome editing

被引：37

作者：

Imai, Ryozo ^{[1
]}

Hamada, Haruyasu ^{[2
]}

Liu, Yuelin ^{[1
]}

Linghu, Qianyan ^{[1
]}

Kumagai, Yuya ^{[1
]}

Nagira, Yozo ^{[2
]}

Miki, Ryuji ^{[2
]}

Taoka, Naoaki ^{[2
]}

机构：

[1] Natl Agr & Food Res Org NARO, Inst Agrobiol Sci, 1-2 Owashi, Tsukuba, Ibaraki 3058634, Japan

[2] KANEKA CORP, Biotechnol Res Labs, Pharma & Supplemental Nutr Solut Vehicle, Takasago, Hyogo 6768688, Japan

来源：

PLANT BIOTECHNOLOGY | 2020年 / 37卷 / 02期

关键词：

biolistic; genome editing; SAM; wheat; AGROBACTERIUM-MEDIATED TRANSFORMATION; SOYBEAN GLYCINE-MAX; FLORAL-DIP; STABLE TRANSFORMATION; EFFICIENT; WHEAT; DNA; ARABIDOPSIS; EXPRESSION; ERA;

D O I：

10.5511/plantbiotechnology.20.0206a

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transformation is a key step in modern breeding technology that involves genome editing. The requirement for in vitro tissue culture and regeneration hampers application of this technology to commercially important varieties of many crop species. To overcome this problem, we developed a simple and reproducible in planta transformation method in wheat (Tritticum aestivum L.). Our in planta particle bombardment (iPB) method utilizes the shoot apical meristem (SAM) as a target tissue. The SAM contains a subepidermal cell layer termed L2, from which germ cells later develop during floral organogenesis. The iPB method can also be used for genome editing through transient CRISPR/Cas9 expression or direct delivery of the CRISPR/Cas9 ribonucleoprotein. In this review, we describe the iPB technology and provide an overview of its current and future applications in plant transformation and genome editing.

引用

页码：171 / 176

页数：6

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