Endothelial cells derived from fetal bovine aorta (BAECs) undergo apoptosis in three-dimensional (3-D) type I collagen lattice in the absence of specific angiogenic factor. In the presence of angiogenic factor, BAECs survive and form a capillary-like tube structure in 3-D culture. In the present study we elucidate the mechanisms of BAECs apoptosis or survival and tube formation in 3-D culture, When BAECs embedded in collagen lattice were cultured with angiogenic factor (fibroblast growth factor-a (FGF-S) or 4 beta-phorbol la-myristate 13-acetate (PMA)) in the presence of PD98059, a specific inhibitor of mitogen-activated protein kinase kinase, BAECs did not form tube structures and underwent apoptosis in collagen lattice. Function-blocking antibody against alpha v beta 3 integrin also inhibited tube formation and induced apoptosis in 3-D culture in the presence of angiogenic factors. Exposure of BAECs to FGF-S and PMA had no effect on the (alpha v beta 3 integrin expression but induced the activation of alpha v beta 3 integrin. PD98059 attenuated alpha v beta 3 integrin activation in response to angiogenic factor. KB-R8301, a hydroxamic acid-based matrix metalloproteinase (MMP) inhibitor, prevented apoptotic cell death in the absence of angiogenic factor in 3-D culture and enhanced capillary-like tube formation in the presence of angiogenic factor, which was not inhibited by the anti-alpha v beta 3 integrin antibody. The results suggest that angiogenic factor-induced alpha v beta 3 integrin activation through the MER-ERK pathway regulates the BAEC fate between apoptosis and angiogenesis in collagen lattice. MMP derived from BAECs seems to play a key role in the release of cryptic ligands for alpha v beta 3 integrin from intact collagen. (C) 1999 Academic Press.
机构:
Seikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, JapanSeikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, Japan
Iuchi, Katsuya
Ema, Mika
论文数: 0引用数: 0
h-index: 0
机构:
Seikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, JapanSeikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, Japan
Ema, Mika
Suzuki, Moe
论文数: 0引用数: 0
h-index: 0
机构:
Seikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, JapanSeikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, Japan
Suzuki, Moe
Yokoyama, Chikako
论文数: 0引用数: 0
h-index: 0
机构:
Yamagata Univ, Dept Biochem Engn, Grad Sch Sci & Engn, Yonezawa, Yamagata 9928510, JapanSeikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, Japan
Yokoyama, Chikako
Hisatomi, Hisashi
论文数: 0引用数: 0
h-index: 0
机构:
Seikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, JapanSeikei Univ, Dept Mat & Life Sci, Fac Sci & Technol, 3-3-1 Kichijojikitamachi, Tokyo 1808633, Japan
机构:
Chung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Lin, Chin-Yin
Hsieh, Yih-Shou
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ Hosp, Clin Lab, Taichung, Taiwan
Chung Shan Med Univ, Sch Med, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Hsieh, Yih-Shou
Chu, Shu-Chen
论文数: 0引用数: 0
h-index: 0
机构:
Cent Taiwan Univ Sci & Technol, Inst & Dept Food Sci, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Chu, Shu-Chen
Hsu, Li-Sung
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Hsu, Li-Sung
Huang, Shih-Chien
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Dept Hlth Ind Technol Management, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Huang, Shih-Chien
Chen, Pei-Ni
论文数: 0引用数: 0
h-index: 0
机构:
Chung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
Chung Shan Med Univ Hosp, Clin Lab, Taichung, TaiwanChung Shan Med Univ, Inst Med, 110,Sect 1,Chien Kuo N Rd, Taichung 402, Taiwan
机构:
Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Park, Ok-Jin
Kim, A. Reum
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Kim, A. Reum
So, Yoon Ju
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
So, Yoon Ju
Im, Jintaek
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Im, Jintaek
Ji, Hyun Jung
论文数: 0引用数: 0
h-index: 0
机构:
Korea Atom Energy Res Inst, Res Div Radiat Sci, Jeongeup, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Ji, Hyun Jung
Ahn, Ki Bum
论文数: 0引用数: 0
h-index: 0
机构:
Korea Atom Energy Res Inst, Res Div Radiat Sci, Jeongeup, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Ahn, Ki Bum
Seo, Ho Seong
论文数: 0引用数: 0
h-index: 0
机构:
Korea Atom Energy Res Inst, Res Div Radiat Sci, Jeongeup, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seo, Ho Seong
Yun, Cheol-Heui
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Agr Biotechnol, Seoul, South Korea
Seoul Natl Univ, Inst Green Bio Sci & Technol, Pyeongchang, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Yun, Cheol-Heui
Han, Seung Hyun
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea
Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul, South KoreaSeoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, Seoul, South Korea