Purification, characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia

被引：90

作者：

Li, Shi-Chao ^{[1
]}

Yang, Xiao-Ming ^{[1
]}

Ma, Hai-Le ^{[1
]}

Yan, Jing-Kun ^{[1
]}

Guo, Dan-Zhao ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2015年 / 133卷

基金：

中国国家自然科学基金;

关键词：

Phellinus igniarius mycelia; Polysaccharide; Purification; Antitumor activity; LINTEUS; MUSHROOM; CULTURE;

D O I：

10.1016/j.carbpol.2015.07.013

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Water-soluble intracellular polysaccharides (IPS) were extracted from cultured mycelia of Phellinus igniarius. The IPS were purified by ethanol fractional precipitation, ion-exchange and size exclusion chromatography in that order. Homogeneous polysaccharide IPSW-1, IPSW-2, IPSW-3, and IPSW-4 were obtained, which molecular characteristics were examined using multiangle laser-light scattering and refractive index detector system. The average molecular weights of them were 34.1, 17.7, 15.1, 21.7 kDa, respectively. GC analysis indicated that IPSW-1, IPSW-2 and IPSW-3 all only contained glucose, while IPSW-4 was composed of rhamnose, xylose, mannose, glucose and galactose in a molar ratio of 1.29:1.21:1:43.86:1.86. UV and IR analysis suggested they belonged to alpha-type of the pyran group and didn't contain protein. These homogeneous polysaccharides could inhibit the growth of SW480 and HepG2 cells to a certain extent in a dose-dependent manner. So they could be beneficial for the further development of a natural carcinoma preventive agent and functional food. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：24 / 30

页数：7