A reliable method for extraction of RNA from various conifer tissues

被引：46

作者：

Dong, JZ ^{[1
]}

Dunstan, DI ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON,SK S7N 0W9,CANADA

来源：

PLANT CELL REPORTS | 1996年 / 15卷 / 07期

关键词：

conifer; Picea glauca; RNA extraction;

D O I：

10.1007/BF00232985

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 mu g up to 700 mu g total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.

引用

页码：516 / 521

页数：6

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