DEVELOPMENT OF CHLOROPLAST MICROSATELLITE MARKERS FOR PHYLOGENETIC ANALYSIS IN BRASSICACEAE

被引：7

作者：

Awasthi, Praveen ^{[1
]}

Ahmad, Irshad ^{[1
]}

Gandhi, Sumit G. ^{[1
]}

Bedi, Yashbir S. ^{[1
]}

机构：

[1] CSIC, Indian Inst Integrat Med, Plant Biotechnol Div, Jammu 180001, India

来源：

ACTA BIOLOGICA HUNGARICA | 2012年 / 63卷 / 04期

关键词：

Simple Sequence Repeat; SSRs; chloroplast DNA; universal primers; in silico; SIMPLE SEQUENCE REPEATS; GENOME VISUALIZATION; ORGANELLAR GENOMES; PRIMERS; EVOLUTION; REGIONS; SET; AMPLIFICATION; DIVERSITY; PATTERNS;

D O I：

10.1556/ABiol.63.2012.4.5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

By employing in silico tools, we devised new chloroplast microsatellite primers for inferring phylogenetic relationships within Brassicaceae. Microsatellite repeats were scanned in 12 chloroplast genomes of Brassicaceae, regions flanking these repeats were aligned and 19 universal primers were designed. Fifteen of these primer pairs are predicted to yield polymorphic amplicons, that are more or less evenly distributed throughout the chloroplast genomes. Finally, using PCR, we have validated three primer pairs on a limited 'test set' of plants, different from those used in computational analysis.

引用

页码：463 / 473

页数：11

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