CO-CULTURE OF A BRAIN ORGANOID DERIVED FROM HUMAN IPSCS AND VASCULATURE ON A CHIP

被引：0

作者：

Isshiki, Yohei ^{[1
]}

Kaneko, Taikopaul ^{[1
]}

Tamada, Atsushi ^{[2
]}

Muguruma, Keiko ^{[2
]}

Yokokaw, Ryuji ^{[1
]}

机构：

[1] Kyoto Univ, Kyoto, Japan

[2] Kansai Med Univ, Hirakata, Osaka, Japan

来源：

2020 33RD IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2020) | 2020年

关键词：

Brain organoid; Vasculature; Microfluidic device; Organ-on-a-chip; CELLS; MODEL;

D O I：

10.1109/mems46641.2020.9056422

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this study, we developed a new system to co-culture human induced pluripotent stem cells (hiPSCs)-derived brain organoids and vasculature made of HUVECs in a microfluidic device. Our device can make perfusable vascular networks in a microchannel. We firstly optimized the type of media to co-culture hiPSCs-derived brain organoids and HUVECs. Then, the effect of vasculature on the differentiation of brain organoids was evaluated by our new culture system. We found that on-chip vasculature promoted neural differentiation and maturation as compared to the conventional mono-culture. This brain organoids-on-a-chip system would be a powerful tool for various stem cell-based organoids engineering and its application in developmental biology and human disease studies.

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页码：1024 / 1027

页数：4

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