A highly sensitive and specific real-time PCR assay for the detection of spotted fever and typhus group rickettsiae

被引：236
作者：
Stenos, J [1 ]
Graves, SR [1 ]
Unsworth, NB [1 ]
机构：
[1] Univ Melbourne, Australian Rickettsial Ref Lab, Geelong Hosp, Dept Clin & Biomed Sci, Geelong, Vic 3220, Australia
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2005年 / 73卷 / 06期
关键词：
D O I：
10.4269/ajtmh.2005.73.1083
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A highly specific real-time polymerase chain reaction (PCR) assay was developed to detect spotted fever and typhus group rickettsiae using the citrate synthase gene as the target. The assay amplified rickettsial members of the spotted fever and typhus group including Rickettsia akari, R. australis, R. conorii, R. honei, "R. marmionii, " R. sibirica, R. rickettsii, R. typhi, and R. prowazekii. The ancestral group rickettsia, R. bellii, did not produce a positive reaction, nor did other members of the order Rickettsiales or any non-rickettsial bacteria. The assay had a sensitivity of one target copy number per reaction as determined by serial dilutions of a plasmid containing a spotted fever group target sequence. This quantitative assay is useful for the enumeration of rickettsiae in clinical specimens and the diagnosis of rickettsial illnesses, when rickettsial numbers are very low.
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页码：1083 / 1085
页数：3
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