Pandemic 2009 H1N1 Influenza A Virus Carrying a Q136K Mutation in the Neuraminidase Gene Is Resistant to Zanamivir but Exhibits Reduced Fitness in the Guinea Pig Transmission Model

被引：20

作者：

Kaminski, Michael M. ^{[1
]}

Ohnemus, Annette ^{[1
]}

Staeheli, Peter ^{[1
]}

Rubbenstroth, Dennis ^{[1
]}

机构：

[1] Univ Freiburg, Dept Virol, D-79106 Freiburg, Germany

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 03期

关键词：

IN-VITRO; OSELTAMIVIR RESISTANCE; HEALTHY-VOLUNTEERS; A(H1N1) VIRUSES; ACTIVE-SITE; INHIBITORS; PHARMACOKINETICS; SENSITIVITY; VARIANTS; VIVO;

D O I：

10.1128/JVI.02507-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Resistance of influenza A viruses to neuraminidase inhibitors can arise through mutations in the neuraminidase (NA) gene. We show here that a Q136K mutation in the NA of the 2009 pandemic H1N1 virus confers a high degree of resistance to zanamivir. Resistance is accompanied by reduced numbers of NA molecules in viral particles and reduced intrinsic enzymatic activity of mutant NA. Interestingly, the Q136K mutation strongly impairs viral fitness in the guinea pig transmission model.

引用

页码：1912 / 1915

页数：4

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