Investigation of Anti-oxidative and Anti-genotoxic Effects of Origanum vulgare L. Essential Oil on Human Lymphocytes In vitro

被引:11
作者
Ceker, Selcuk [1 ]
Agar, Guleray [2 ]
Nardemir, Gokce [2 ]
Anar, Mustafa [3 ]
Kizil, Hamit Emre [4 ]
Alpsoy, Lokman [5 ]
机构
[1] Agri Ibrahim Univ, Cent Res & Applicat Labs, TR-100 Agri, Turkey
[2] Ataturk Univ, Dept Biol, Fac Sci, TR-25240 Erzurum, Turkey
[3] Ataturk Univ, Dept Biol Educ, Kazzm Karabekir Fac Educ, TR-25240 Erzurum, Turkey
[4] Bayburt Univ, Fac Bayburt, Dept Sci Teacher Training, Bayburt, Turkey
[5] Fatih Univ, Fac Art & Sci, Dept Biol, Istanbul, Turkey
关键词
Aflatoxin B-1; anti-genotoxicity; antioxidant enzymes; Origanum vulgare; EASTERN ANATOLIA REGION; PLANT ESSENTIAL OILS; METHANOL EXTRACT; ANTIBACTERIAL; CULTURES; GLUTATHIONE; ANTIFUNGAL; STRAINS; GROWTH; MODEL;
D O I
10.1080/0972060X.2012.10662604
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Origanum vulgare L. (Lamiaceae) is a flavoring herb widely using around the world. Some preparations and extracts from O. vulgare have been used in Turkish traditional medicine to treat several ailments including digestive disorders and menstrual problems. This study is designed to examine the chemical composition and in vitro antigenotoxic and antioxidant activity of the essential oil of O. vulgare (OVE) from Turkey. The essential oil of O. vulgare was analized by using GC. OVEs that were investigated antagonistic effects against oxidative stress and the genotoxic effects of aflatoksin B-1 (AFB(1)). Anti-genotoxic activity was evaluated by sister chromatid exchanges (SCEs) and micronucleus (MN) tests. In additional to investigating protective role of OVEs against the oxidative stress of AFB(1) in vitro, oxidative status was assessed on erythrocytes by measuring following oxidative stress markers like; super oxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA). The total of the 25 identified components are accounted for 96.03 % (GC). Major components of the oil were beta-caryophyllene (20.40 %), and caryophyllene oxide (34.44 %). In the SCE and MN tests, it was observed that OVEs suppressed the mutagenic effects of AFB(1). OVEs were observed to modulate the adverse effects of AFB(1). The results of this experiment clearly reveal that OVEs have strong anti-oxidative and anti-genotoxic effect. These anti-genotoxic effects of O. vulgare essential oils are probably related to its action on the enzymatic activation system. Anti-oxidative and anti-genotoxic effect may be role of these compounds (beta-caryophyllene and caryophyllene oxide).
引用
收藏
页码:997 / 1005
页数:9
相关论文
共 47 条
[1]   Pennyroyal toxicity: Measurement of toxic metabolite levels in two cases and review of the literature [J].
Anderson, IB ;
Mullen, WH ;
Meeker, JE ;
KhojastehBakht, SC ;
Oishi, S ;
Nelson, SD ;
Blanc, PD .
ANNALS OF INTERNAL MEDICINE, 1996, 124 (08) :726-+
[2]  
[Anonymous], 2004, RIRDC PUBLICATION
[3]   The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. [J].
Baher, ZF ;
Mirza, M ;
Ghorbanli, M ;
Rezaii, MB .
FLAVOUR AND FRAGRANCE JOURNAL, 2002, 17 (04) :275-277
[4]   Cytotoxicity and gene induction by some essential oils in the yeast Saccharomyces cerevisiae [J].
Bakkali, F ;
Averbeck, S ;
Averbeck, D ;
Zhiri, A ;
Idaomar, A .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2005, 585 (1-2) :1-13
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]  
Bruneton J., 1999, MED PLANTS ESSENTIAL
[7]   Antimutagenicity of Stevia pilosa and Stevia eupatoria evaluated with the Ames test [J].
Carino-Cortes, R. ;
Hernandez-Ceruelos, A. ;
Torres-Valencia, J. M. ;
Gonzalez-Avila, A. ;
Arriaga-Alba, M. ;
Madrigal-Bujaidar, E. .
TOXICOLOGY IN VITRO, 2007, 21 (04) :691-697
[8]   PRODUCTION OF MICRONUCLEI FROM CHROMOSOME-ABERRATIONS IN IRRADIATED CULTURES OF HUMAN LYMPHOCYTES [J].
COUNTRYMAN, PI ;
HEDDLE, JA .
MUTATION RESEARCH, 1976, 41 (2-3) :321-331
[9]   Antibacterial activity of the essential oil of Origanum vulgare L. (Lamiaceae) against bacterial multiresistant strains isolated from nosocomial patients [J].
da Costa, Adalberto Coelho ;
Cavalcanti dos Santos, Bernadete Helena ;
Santos Filho, Lauro ;
Lima, Edeltrudes de Oliveira .
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, 2009, 19 (1B) :236-241
[10]   The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp and Clavibacter michiganensis subsp michiganensis [J].
Daferera, DJ ;
Ziogas, BN ;
Polissiou, MG .
CROP PROTECTION, 2003, 22 (01) :39-44