Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

被引：116

作者：

Loss-Morais, Guilherme ^{[1
]}

Waterhouse, Peter M. ^{[2
]}

Margis, Rogerio ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Porto Alegre, RS, Brazil

[2] Univ Sydney, Sydney, NSW 2006, Australia

来源：

BIOLOGY DIRECT | 2013年 / 8卷

关键词：

tRNAs; Small RNA; tRFs; tRNA-derived RNA fragments; Argonaute; Arabidopsis; ARABIDOPSIS;

D O I：

10.1186/1745-6150-8-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.

引用

页数：5

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