Establishment and Quality Assessment of Tissue Cultures in Glycyrrhiza uralensis Fisch.

被引：15

作者：

Wang, Juan ^{[1
]}

Gao, Wenyuan ^{[1
]}

Zhang, Liming ^{[2
]}

Huang, Luqi ^{[3
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China

[2] Tianjin Univ Sci & Technol, Inst Biol Engn Tradit Chinese Med, Tianjin 300457, Peoples R China

[3] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2013年 / 169卷 / 02期

关键词：

Glycyrrhiza uralensis Fisch; Tissue culture; Glycyrrhizic acid; Flavonoids; Cluster analysis;

D O I：

10.1007/s12010-012-0012-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, seedling, callus, cell, and adventitious root of Glycyrrhiza uralensis Fisch. have been established. In order to find the best one for producing G. uralensis active constituents, triterpenoid saponins and flavonoids in native root and tissue cultures were determined, and the contents in different G. uralensis materials were analyzed using cluster analysis. The contents of triterpenoid saponins and glycyrrhizic acid in tissue cultures were much lower than that in native G. uralensis. The total flavonoids content we determined in adventitious root was 6.31 mg g(-1), which was close to that of native root (9.82 mg g(-1)). Based on the cluster analysis, we found that G. uralensis cultures were not suitable for production of glycyrrhizic acid, while adventitious root had a greater capability of flavonoids production comparing to seedling, callus, and cell.

引用

页码：588 / 594

页数：7

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